CD22 MicroBeads, human

CD22 MicroBeads were developed for positive selection or depletion of human B cells from PBMCs, bone marrow, or single-cell suspensions from lymphoid tissues.

Data and images for CD22 MicroBeads, human

Figures

Figure 1

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Separation of CD22
+
cells from PBMCs using CD22 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD19-FITC.

Figure 1

Separation of CD22
+
cells from PBMCs using CD22 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD19-FITC.

Specifications for CD22 MicroBeads, human

Overview

CD22 MicroBeads were developed for positive selection or depletion of human B cells from PBMCs, bone marrow, or single-cell suspensions from lymphoid tissues.

Detailed product information

Background information

In peripheral blood, cell surface expression of the CD22 antigen is found on mature B cells, but is lost during terminal differentiation to plasma cells. In bone marrow, surface expression of CD22 can be detected from the pre-B cell stage on. Cytoplasmic expression of CD22 can also be found in late pro-B cells and early pre-B cells.

Downstream applications

The purified B cells may be used for cell biological studies, such as analysis of signal transduction
2
, mixed lymphocyte responses
1
, antigen presentation, or for molecular studies, e.g. PCR analysis
3
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD22 MicroBeads, human

Publications

  1. Butch, A. W. et al. (1999) Human follicular dendritic cells inhibit superantigen-induced T-cell proliferation by distinct mechanisms. Blood 94: 216-224
  2. Jacquot, S. et al. (2001) B cell co-receptors regulating T cell-dependent antibody production in common variable immunodeficiency: CD27 pathway defects identify subsets of severely immuno-compromised patients. Int. Immunol. 13: 871-876
  3. Borson, N. D. et al. (2002) Altered mRNA expression of Pax5 and Blimp-1 in B cells in multiple myeloma. Blood 100: 4629-4639

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