Clone:
REA1087
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
MICS, IF, IHC
Alternative names:
CD20cy, CD20 cytoplasmic domain

Specifications for CD20 Cytoplasmic Antibody, anti-human, REAfinity™

Overview

Clone REA1087 recognizes an intracytoplasmic epitope localized on the CD20 antigen, which is a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca2
+
channel and might function in the regulation of local responses during B cell activation. In vitro effects of CD20-specific antibodies on resting B cells indicate that CD20 is able to transduce an extracellular signal affecting the G0/G1 cell cycle transition. Studies have demonstrated that CD20-initiated intracellular signals involve tyrosine kinase activation and that CD20 is tightly associated with both serine and tyrosine kinases.
Additional information: Clone REA1087 displays negligible binding to Fc receptors.

Alternative names

CD20cy, CD20 cytoplasmic domain

Detailed product information

Technical specifications

CloneREA1087
Clonalitymonoclonal
Isotyperecombinant human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
Cross-reactivitymouse
AntigenCD20 Cytoplasmic
Alternative names of antigenCD20cy, CD20 cytoplasmic domain
RRIDAB_2857426, AB_2857425

References for CD20 Cytoplasmic Antibody, anti-human, REAfinity™

Publications

  1. Einfeld, D. A. et al. (1988) Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains. EMBO J. 7(3): 711-717
  2. Deans, J. P. et al. (1995) Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J. Biol. Chem. 270(38): 22632-22638
  3. Vaughan, A. T. et al. (2015) Activatory and inhibitory Fcγ receptors augment rituximab-mediated internalization of CD20 independent of signaling via the cytoplasmic domain. J. Biol. Chem. 290(9): 5424-5437

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