Clone:
REA972
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
LFA-2, SRBC-R, T11, SRBC

Extended validation for CD2 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA972
RPA-2.10++
LT2++
S5.2++
Cells were incubated with an excess of purified unconjugated CD2 (REA972) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD2 (REA972). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD2 (REA972). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD2 (REA972). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD2 Antibody, anti-human, REAfinity™

Overview

Clone REA972 recognizes the human CD2 antigen, a 50 kDa single-chain transmembrane glycoprotein also known as LFA-2 or receptor for sheep erythrocytes. CD2 functions as a costimulatory molecule on T and natural killer (NK) cells and is a receptor for other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58). It belongs to the Ig superfamily and is involved in cell signaling and lymphocyte adhesion. The CD2 antibody reacts with 80–90% of peripheral blood lymphocytes and more than 95% of thymocytes and recognizes all T cells and a subset of NK cells.
Additional information: Clone REA972 displays negligible binding to Fc receptors.

Alternative names

LFA-2, SRBC-R, T11, SRBC

Detailed product information

Technical specifications

CloneREA972
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
,
squirrel monkey (
Saimiri sciureus
)
AntigenCD2
Alternative names of antigenLFA-2, SRBC-R, T11, SRBC
Molecular mass of antigen [kDa]37
Distribution of antigenB cells, lymphocytes, macrophages, mast cells, NK cells, T cells, thymocytes
Entrez Gene ID914
RRIDAB_2727364, AB_2727429, AB_2727365, AB_2727430, AB_2727366, AB_2727433, AB_2727369, AB_2727431, AB_2727367, AB_2727432, AB_2727368, AB_2801924, AB_2727428

Resources for CD2 Antibody, anti-human, REAfinity™

References for CD2 Antibody, anti-human, REAfinity™

Publications

  1. Sanchez-Madrid, F. et al. (1982) Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. U.S.A. 79: 7489-7493
  2. Ozwara, H. et al. (1997)
    Flow cytometric analysis on reactivity of human T lymphocyte-specific and cytokine-receptor-specific antibodies with peripheral blood mononuclear cells of chimpanzee (
    Pan troglodytes
    ), rhesus macaque (
    Macacca mulatta
    ) and squirrel monkey (
    Saimiri sciureus
    ).
    J. Med. Primatol. 26(3): 164-171

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