CD197 (CCR7) Antibody, anti-human

Name: CD197 (CCR7) Antibody, anti-human
Availability: InStock

CD197 (CCR7) Antibody, anti-humanExtended Validation

Clone:

FR 11-11E8

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ

Applications:

FC

Alternative names:

CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Flow cytometry applications forCD197 (CCR7) Antibody, anti-human

PE
pure

Conjugate:

PE

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-120-604, 130-120-464

Protocols:

Cell surface flow cytometry staining protocol for CCRs (1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies as well as with CD4 antibodies and CD45RA antibodies. Cells were analyzed by flow cytometry and gated on CD4⁺ T cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for CD197 (CCR7) Antibody, anti-human

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (FR 11-11E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (FR 11-11E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD197 (CCR7) Antibody, anti-human

Overview

CD197, also known as chemokine (C-C motif) receptor 7 (CCR7), is a chemokine receptor with a C-C motif that mediates homing of T cells to secondary lymphoid organs via high endothelial venules (HEV). For example, naive T cells migrate very efficiently through lymph nodes using the adhesion molecules L-selectin, CD62L, and CCR7. Ligands for CCR7, e.g. CCL19, are expressed by the HEV of secondary lymphoid organs, by parenchymal cells within T cell zones of lymph nodes, and by endothelial cells at the openings of lymphatic vessels within peripheral tissues. Expression of CCR7 and the CD45RA isoform distinguishes three subsets of T cells: naive T cells (CCR7

⁺

CD45RA

⁺

), central memory T cells (CCR7

⁺

CD45RA

^–

), and effector memory T cells (CCR7

^–

CD45RA

^–

).

Alternative names

CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Detailed product information

Technical specifications

Clone	FR 11-11E8
Clonality	monoclonal
Isotype	mouse IgG1κ
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD197 (CCR7)
Alternative names of antigen	CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1
Molecular mass of antigen [kDa]	40
Distribution of antigen	B cells, dendritic cells, macrophages, NK cells, T cells, thymocytes
Entrez Gene ID	1236
RRID	AB_2801767, AB_10828660, AB_2801776

Resources for CD197 (CCR7) Antibody, anti-human

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CD197 (CCR7) Antibody, anti-human

Publications

Sallusto, F. et al. (1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401: 708-712

Applications

Extended validation