Clone:
FR 11-11E8
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Extended validation for CD197 (CCR7) Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (FR 11-11E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (FR 11-11E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (FR 11-11E8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD197 (CCR7) Antibody, anti-human

Overview

CD197, also known as chemokine (C-C motif) receptor 7 (CCR7), is a chemokine receptor with a C-C motif that mediates homing of T cells to secondary lymphoid organs via high endothelial venules (HEV). For example, naive T cells migrate very efficiently through lymph nodes using the adhesion molecules L-selectin, CD62L, and CCR7. Ligands for CCR7, e.g. CCL19, are expressed by the HEV of secondary lymphoid organs, by parenchymal cells within T cell zones of lymph nodes, and by endothelial cells at the openings of lymphatic vessels within peripheral tissues. Expression of CCR7 and the CD45RA isoform distinguishes three subsets of T cells: naive T cells (CCR7
+
CD45RA
+
), central memory T cells (CCR7
+
CD45RA
), and effector memory T cells (CCR7
CD45RA
).

Alternative names

CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Detailed product information

Technical specifications

CloneFR 11-11E8
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD197 (CCR7)
Alternative names of antigenCCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1
Molecular mass of antigen [kDa]40
Distribution of antigenB cells, dendritic cells, macrophages, NK cells, T cells, thymocytes
Entrez Gene ID1236
RRIDAB_2801767, AB_10828660, AB_2801776

Resources for CD197 (CCR7) Antibody, anti-human

References for CD197 (CCR7) Antibody, anti-human

Publications

  1. Sallusto, F. et al. (1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401: 708-712

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