Clone:
REA190
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC, MC
Alternative names:
CCR6, BN-1, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22

Extended validation for CD196 (CCR6) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA190
11A9+
R6H1++
TG7/CCR6++
G034E3+
Cells were incubated with an excess of purified unconjugated CD196 (CCR6) (REA190) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD196 (CCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD196 (CCR6) antibodies and with a suitable counterstaining. As a control, CD196 (CCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD196 (CCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD196 (CCR6) antibodies and with a suitable counterstaining. As a control, CD196 (CCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD196 (CCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD196 (CCR6) antibodies and with a suitable counterstaining. As a control, CD196 (CCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD196 (CCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD196 (CCR6) antibodies and with a suitable counterstaining. As a control, CD196 (CCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD196 (CCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD196 (CCR6) antibodies and with a suitable counterstaining. As a control, CD196 (CCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD196 (CCR6). Human peripheral blood mononuclear cells (PBMCs) were stained with CD196 (CCR6) antibodies and with a suitable counterstaining. As a control, CD196 (CCR6) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD196 (CCR6) (REA190). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD196 (CCR6) (REA190). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD196 (CCR6) (REA190). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD196 (CCR6) Antibody, anti-human, REAfinity™

Overview

Clone REA190 recognizes CD196 (CCR6), a seven transmembrane G protein–coupled receptor, which belongs to the family of beta chemokine receptors. Expression of CD196 is found on T cells, naive and memory B cells, and immature dendritic cells (DCs). CD196 selectivly binds chemokine MIP-3α/CCL20, which is secreted by a number of cells and tissues. In addition, members of a family of small cationic antimicrobial peptides, such as human β-defensins (hBD)-1 and -2, which are released by epithelial cells in response to microbial invasion or proinflammatory stimuli, are also shown to bind to CCR6. This interaction induce chemotaxis in immature dendritic cells and memory T cells.
Additional information: Clone REA190 displays negligible binding to Fc receptors.

Alternative names

CCR6, BN-1, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22

Detailed product information

Technical specifications

CloneREA190
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD196 (CCR6)
Alternative names of antigenCCR6, BN-1, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22
Molecular mass of antigen [kDa]42
Distribution of antigenB cells, T cells, dendritic cells, T cells, B cells, dendritic cells
Entrez Gene ID1235
RRIDAB_2733354, AB_2733210, AB_2733211, AB_2752157, AB_2752106, AB_2801775, AB_2801766, AB_2784042, AB_2784041, AB_2655940, AB_2733353

Resources for CD196 (CCR6) Antibody, anti-human, REAfinity™

References for CD196 (CCR6) Antibody, anti-human, REAfinity™

Publications

  1. Yang, D. et al. (1999) Beta-defensins: Linking innate and adaptive immunity through dendritic and T cell CCR6. Science 286(5439): 525-528
  2. Bergmann-Leitner, E. et al. (2018) Identification of immune signatures of novel adjuvant formulations using machine learning. Sci. Rep. 8(1): 17508
  3. Dorraji, E. et al. (2018) Mesenchymal stem cells and T cells in the formation of tertiary lymphoid structures in lupus nephritis. Sci. Rep. : 7861
  4. Farooq, F. et al. (2016) Circulating follicular T helper cells and cytokine profile in humans following vaccination with the rVSV-ZEBOV Ebola vaccine. Sci. Rep. 6: 27944
  5. Schutyser, E. et al. (2003) The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 14(5): 409-426
  6. Armas-González, E. et al. (2018) Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis. Arthritis Res. Ther. 20(1): 114

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