CD19 MicroBeads were developed for positive selection or depletion of CD19
+
B cells from different cell sources such as human PBMCs, bone marrow, lymphoid tissues, or cell cultures.

Data and images for CD19 MicroBeads, human

Figures

Figure 1

CD19
+
cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
After separation
View details

Figure 1

CD19
+
cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD19
+
cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

View details
Separation of CD19
+
cells from PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Figure 2

Separation of CD19
+
cells from PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Specifications for CD19 MicroBeads, human

Overview

CD19 MicroBeads were developed for positive selection or depletion of CD19
+
B cells from different cell sources such as human PBMCs, bone marrow, lymphoid tissues, or cell cultures.

Detailed product information

Background information

As a B cell lineage marker, the CD19 antigen is expressed from the early pro-B cell stage to the B cell lymphoblast stage, but the expression is down-regulated upon B cell maturation to plasma cells. The CD19 antigen is further expressed on most malignant B cells and on a subset of follicular dendritic cells.

Downstream applications

Isolated CD19
+
B cells were used for studies on immunoglobulin production, such as IgE synthesis
1, 2
and secretion
3
, chemotaxis assays
4
, or mixed lymphocyte reactions. Positively selected CD19
+
B cells were also used for molecular studies such as gene expression profiling.
5
For the analysis of minimal residual diseases in B cell malignancies, enrichment of B cells increases the sensitivity of detection.
6

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD19 MicroBeads, human

Reviews for CD19 MicroBeads, human

Anti-human CD19 Microbeads - A Simple Method to Obtain Highly Pure B Cells from PBMC

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CD19 MicroBeads, human (130-050-301)

The primary goal of our research was to obtain highly pure CD19+ B cells, as well as other immune cell types, from human peripheral blood mononuclear cells. The cells were required to be highly pure since downstream analysis involved microarray analysis of gene expression. This product was selected over competing products in part due to its ease of use and the previously established and protocols provided by the manufacturer.

Human CD19 Separation Beads

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CD19 MicroBeads, human (130-050-301)

We are interested in antibody responses that protect against HIV infection, and engineering B cells to express bNAb. We use a humanized mouse model consisting of activated PBMC injected into NSG mouse spleens. We have been setting up experiments where we transduce B cells with lentiviral vectors expressing different bNAb vectors and injected them into the mice to investigate their protective capacity. We purify B cells using these beads.

References for CD19 MicroBeads, human

Publications

  1. Aebischer et al. (1994)
    Neuropeptides are potent modulators of human
    in vitro
    immunoglobulin E synthesis.
    Eur. J. Immunol. 24: 1908-1913
  2. Stämpfli et al. (1994) Inhibition of human IgE synthesis by anti-IgE antibodies requires divalent recognition. Eur. J. Immunol. 24: 2161-2167
  3. Zürcher, A. W. et al. (1995) IgE-producing hybridomas established after B-cell culture in the CD40 system. Immunol. Lett. 46: 49-57
  4. Schratzberger, P. et al. (1997) Differential chemotactic activities of sensory neuropeptides for human peripheral blood mononuclear cells. J. Immunol. 158: 3895-3901
  5. Klein, U. et al. (2001) Gene expression profiling of B cell chronic lymphocytic leukemia reveals a homogeneous phenotype related to memory B cells. J. Exp. Med. 194: 1625-1638
  6. Pugh, R. E. et al. (1998) CD19 selection improves the sensitivity of B cell lymphoma detection. J. Hematother. 7: 159-168

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