Clone:
JJC11.6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
KIR2DS4

Extended validation for CD158i (KIR2DS4) Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD158i (KIR2DS4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies and with a suitable counterstaining. As a control, CD158i (KIR2DS4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158i (KIR2DS4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies and with a suitable counterstaining. As a control, CD158i (KIR2DS4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158i (KIR2DS4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies and with a suitable counterstaining. As a control, CD158i (KIR2DS4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158i (KIR2DS4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies and with a suitable counterstaining. As a control, CD158i (KIR2DS4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158i (KIR2DS4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies and with a suitable counterstaining. As a control, CD158i (KIR2DS4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD158i (KIR2DS4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158i (KIR2DS4) antibodies and with a suitable counterstaining. As a control, CD158i (KIR2DS4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158i (KIR2DS4) (JJC11.6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158i (KIR2DS4) (JJC11.6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD158i (KIR2DS4) (JJC11.6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD158i (KIR2DS4) Antibody, anti-human

Overview

Clone JJC11.6 recognizes CD158i (KIR2DS4), a member of the killer immunoglobulin-like receptor (KIR) family expressed on natural killer (NK) cells. CD158i (KIR2DS4) provides an activation signal for NK lytic activity upon interaction with its ligand, HLA-Cw4, in an antigen-independent manner.
KIRs are monomeric receptors possessing high allelic polymorphism with either 2 or 3 Ig-like extracellular domains. The receptor family can be subdivided functionally according to the length of their cytoplasmic tail in inhibitory and activating KIRs.

Alternative names

KIR2DS4

Detailed product information

Technical specifications

CloneJJC11.6
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD158i (KIR2DS4)
Alternative names of antigenKIR2DS4
Molecular mass of antigen [kDa]31
Distribution of antigenNK cells, T cells
Entrez Gene ID3809
RRIDAB_2889460, AB_2660205, AB_871617, AB_2660206, AB_2660207, AB_871618, AB_871616, AB_2889461

Resources for CD158i (KIR2DS4) Antibody, anti-human

Documents and Protocols

App notes/customer reports

Cross-reactivity of KIR antibodies

Certificates

Please follow this
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References for CD158i (KIR2DS4) Antibody, anti-human

Publications

  1. Kratz, G. et al. (2004) MHC Class I-independent recognition of NK-activating receptor KIR2DS4. J. Immunol. 173: 1819-1825
  2. Hsu, K. et al. (2002) The killer cell immunoglobin-like (KIR) genomic region: gene-order, haplotypes and allelic polymorphism. Immunol. Rev. 190: 40-52
  3. Selvakumar, A. et al. (1997) Polymorphism and domain variability of human killer cell inhibitory receptors. Immunol. Rev. 155: 183-196

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