CD138 MicroBeads, mouse

Name: CD138 MicroBeads, mouse
Availability: InStock

CD138 MicroBeads, mouse

CD138 MicroBeads were developed for the isolation of CD138

⁺

cells from bone marrow and spleen.

Product data and images for CD138 MicroBeads, mouse

Figures

Figure 1

Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138

⁺⁺

cells represent the plasma cell fraction in both tissues. CD138

⁺

cells in bone marrow are B cell precursors.

Spleen

A:	B:
Before enrichment	After enrichment
View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.	View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.
Bone marrow

C:	D:
Before enrichment	After enrichment
View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.	View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.

Specifications for CD138 MicroBeads, mouse

Overview

CD138 MicroBeads were developed for the isolation of CD138

⁺

cells from bone marrow and spleen.

Detailed product information

Background information

In mice, CD138 (Syndecan-1) is expressed on pre-B and immature B lymphocytes in the bone marrow. It is lost when B cells emigrate into the periphery, is absent on circulating and peripheral B cells, and is re-expressed upon B cell differentiation into plasmablasts and plasma cells.

Applications

Enrichment of cells expressing mouse CD138 antigen, e.g. plasma cells from bone marrow and spleen.

Columns

MS, LS, or autoMACS

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Columns.

Resources for CD138 MicroBeads, mouse

Data and images