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Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. |
Spleen |
A: | B: |
Before enrichment | After enrichment |
CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. | CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. |
Bone marrow |
C: | D: |
Before enrichment | After enrichment |
CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. | CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. |
Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. |
Spleen |
A: | B: |
Before enrichment | After enrichment |
CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. | CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. |
Bone marrow |
C: | D: |
Before enrichment | After enrichment |
CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. | CD138 MicroBeads, mouseFigure 1Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors. |
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