Name: CD138 MicroBeads, mouse
Availability: InStock

Data and images
Specifications

Product data and images for CD138 MicroBeads, mouse

Figures

Figure 1

Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138

⁺⁺

cells represent the plasma cell fraction in both tissues. CD138

⁺

cells in bone marrow are B cell precursors.

Spleen

A:	B:
Before enrichment	After enrichment
View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.	View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.
Bone marrow

C:	D:
Before enrichment	After enrichment
View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.	View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.

Specifications for CD138 MicroBeads, mouse

Overview

CD138 MicroBeads were developed for the isolation of CD138

⁺

cells from bone marrow and spleen.

Detailed product information

Background information

In mice, CD138 (Syndecan-1) is expressed on pre-B and immature B lymphocytes in the bone marrow. It is lost when B cells emigrate into the periphery, is absent on circulating and peripheral B cells, and is re-expressed upon B cell differentiation into plasmablasts and plasma cells.

Applications

Enrichment of cells expressing mouse CD138 antigen, e.g. plasma cells from bone marrow and spleen.

Columns

MS, LS, or autoMACS

^®

Columns.

Data and images

Data and images for CD138 MicroBeads, mouse

Figures

Figure 1

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138

⁺⁺

cells represent the plasma cell fraction in both tissues. CD138

⁺

cells in bone marrow are B cell precursors.

Spleen

A:	B:
Before enrichment	After enrichment
View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.	View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.
Bone marrow

C:	D:
Before enrichment	After enrichment
View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.	View details Figure 1 Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ⁺⁺ cells represent the plasma cell fraction in both tissues. CD138 ⁺ cells in bone marrow are B cell precursors.

Specifications

Specifications for CD138 MicroBeads, mouse

Overview

CD138 MicroBeads were developed for the isolation of CD138

⁺

cells from bone marrow and spleen.

Detailed product information

Background information

Applications

Enrichment of cells expressing mouse CD138 antigen, e.g. plasma cells from bone marrow and spleen.

Columns

MS, LS, or autoMACS

^®

Columns.

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CD138 MicroBeads, mouse

CD138 MicroBeads, mouse

Product overview

Product data and images for CD138 MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for CD138 MicroBeads, mouse

Overview

Detailed product information

Background information

Applications

Columns

Data and images for CD138 MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for CD138 MicroBeads, mouse

Overview

Detailed product information

Background information

Applications

Columns

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