CD11b MicroBeads were developed for the isolation or depletion of human and mouse cells based on their CD11b expression.

Data and images for CD11b MicroBeads, human and mouse

Figures

Figure 1

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Separation of CD11b
+
cells from human PBMCs using CD11b MicroBeads, an MS Column, and a MiniMACS™ Separator.

Figure 1

Separation of CD11b
+
cells from human PBMCs using CD11b MicroBeads, an MS Column, and a MiniMACS™ Separator.

Figure 2

Positive selection of murine CD11b
+
cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator
Mouse splenocytes before separation
CD11b-depleted splenocytes
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Figure 2

Positive selection of murine CD11b
+
cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator
View details

Figure 2

Positive selection of murine CD11b
+
cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator
Isolated CD11b
+
mouse cells
View details

Figure 2

Positive selection of murine CD11b
+
cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator

Specifications for CD11b MicroBeads, human and mouse

Overview

CD11b MicroBeads were developed for the isolation or depletion of human and mouse cells based on their CD11b expression.

Detailed product information

Background information

In humans, CD11b is strongly expressed on myeloid cells, and weakly expressed on NK cells and some activated lymphocytes.
In mice, the CD11b antigen is expressed on monocytes/macrophages and to a lower extent on granulocytes, NK cells, CD5
+
B1 cells, and a subset of dendritic cells.

Downstream applications

In humans, CD11b MicroBeads can be used for positive selection or depletion of CD11b
+
cells from PBMCs, bone marrow, and other lymphoid tissues. They are mostly used in combination with other MicroBeads for depletion of unwanted cells.
In mice, CD11b MicroBeads have been used to isolate CD11b
+
cells from cell suspensions of spleen
1
, lymph node
2
, peritoneal cavity
3
, liver
4
, muscle tissue
2
, or blood
5
. They were also used in combination with other MicroBeads for the enrichment of mast cells from peritoneal cell suspensions.
3
For the isolation of human monocytes CD14 MicroBeads or the Pan Monocyte Isolation Kit are recommended.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Reviews for CD11b MicroBeads, human and mouse

Beads For Isolation Of Microglia From Mouse Brain

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CD11b MicroBeads, human and mouse (130-049-601)

Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. We have developed a mouse line that is transgenic for some human cell receptors that make mouse cells infectable with HIV. In order to study HIV infection in the brain we isolate microglia from mouse brains and infect the cells in vitro.

Magnetic Beads For Isolation Of CD11b+ Cells

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CD11b MicroBeads, human and mouse (130-049-601)

To prepare bone marrow-derived macrophages, we isolate CD11b+ bone marrow cells from mice to differentiate into macrophages in culture. To do this, we use CD11b microbeads as a reliable, affordable, easy, and quick way to isolate CD11b+ cells from total bone marrow cells.

References for CD11b MicroBeads, human and mouse

Publications

  1. Lohoff, M. et al. (1997)
    Interferon regulatory factor-1 is required for a T helper 1 immune response
    in vivo
    .
    Immunity 6: 681-689
  2. Dupuis, M. et al. (2000) Distribution of DNA vaccines determines their immunogenicity after intramuscular injection in mice. J. Immunol. 165: 2850-2858
  3. Chen, X. J. et al. (1999) Surface and gene expression of immunoglobulin E receptors on mast cells and mast-cell numbers in interleukin-4-gene knockout mice. Immunology 96: 544-550
  4. Do, H. et al. (1999) Expression of factor VIII by murine liver sinusoidal endothelial cells. J. Biol. Chem. 274: 19587-19592
  5. Mathy, N. L. et al. (2000) Cutting edge: CD4 is not required for the functional activity of IL-16. J. Immunol. 164: 4429-4432

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