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Splenocytes from C56BL/6 mice were stained with REAlease CD11b-PerCP (B) or with the corresponding REAL Control (A). REAlease CD11b-PerCP was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD11b-PerCP (D). Cells were labeled with CD115 antibodies in addition and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.
Immunofluorescence analysis of a mouse spleen cryosection fixed with 4% PFA. The section was stained using antigen_conjugate (green) for 10 min at room temperature (A). CD11b Antibody, anti-mouse, PE staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.
Immunofluorescence analysis of a mouse spleen cryosection fixed with ice-cold acetone. The section was stained using CD11b Antibody, anti-mouse, PE (green) for 10 min at room temperature (A). antigen_conjugate staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.
Other clones | Overlap in epitope recognition with REAL113 |
---|---|
M1/70 | ++ |
M1/70.15.11.5 | ++ |
5C6 | + |
REA592 | ++ |
Cells were incubated with an excess of purified unconjugated CD11b (REAL113) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | REAL113 |
---|---|
Clonality | monoclonal |
Isotype control | Control Antibody |
Host | human cell line |
Type of antibody | Releasable antibodies, Primary antibodies, Recombinant antibodies |
Species | mouse |
Cross-reactivity | non-human primate |
Antigen | CD11b |
Alternative names of antigen | integrin αM, Mac-1 α, Integrin alpha-M, CR3A, ITGAM, MAC1A, MO1A, SLEB6 |
Distribution of antigen | myeloid cells, NK cells, microglia, lymphocytes, brain |
RRID | AB_2783918, AB_2811689, AB_2904692 |
Other clones | Overlap in epitope recognition with REAL113 |
---|---|
M1/70 | ++ |
M1/70.15.11.5 | ++ |
5C6 | + |
REA592 | ++ |
Cells were incubated with an excess of purified unconjugated CD11b (REAL113) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | REAL113 |
---|---|
Clonality | monoclonal |
Isotype control | Control Antibody |
Host | human cell line |
Type of antibody | Releasable antibodies, Primary antibodies, Recombinant antibodies |
Species | mouse |
Cross-reactivity | non-human primate |
Antigen | CD11b |
Alternative names of antigen | integrin αM, Mac-1 α, Integrin alpha-M, CR3A, ITGAM, MAC1A, MO1A, SLEB6 |
Distribution of antigen | myeloid cells, NK cells, microglia, lymphocytes, brain |
RRID | AB_2783918, AB_2811689, AB_2904692 |
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