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Human peripheral blood mononuclear cells (PBMCs) were stimulated in vitro for 5 hours with SEB in the presence of monensin and conjugated CD107a (LAMP-1) antibodies. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies. Additionally, cell surface staining was performed with CD8 antibodies. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. CD8+, IFN‑γ–secreting cells are shown.
Other clones | Overlap in epitope recognition with H4A3 |
---|---|
REA792 | ++ |
508921 | + |
Cells were incubated with an excess of purified unconjugated CD107a (LAMP-1) (H4A3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | H4A3 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1κ |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies |
Species | human, non-human primate |
Cross-reactivity | rhesus monkey ( Macaca mulatta) pigtail monkey ( Macaca nemestrina) african green monkey ( Chlorocebus aethiops) chimpanzee ( Pan troglodytes) |
Antigen | CD107a (LAMP-1) |
Alternative names of antigen | LAMPA, LGP120, LAMP-1 |
Molecular mass of antigen [kDa] | 42 |
Distribution of antigen | dendritic cells, endothelial cells, epithelial cells, granulocytes, macrophages, platelets, T cells, neutrophils |
Entrez Gene ID | 3916 |
RRID | AB_2751900, AB_2783907, AB_2783906, AB_2751948, AB_2751901, AB_2751945, AB_2751898, AB_2783905, AB_2783904, AB_2751946, AB_2751899, AB_2801739, AB_2801736, AB_2819409, AB_2819408, AB_2751947 |
Other clones | Overlap in epitope recognition with H4A3 |
---|---|
REA792 | ++ |
508921 | + |
Cells were incubated with an excess of purified unconjugated CD107a (LAMP-1) (H4A3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | H4A3 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1κ |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies |
Species | human, non-human primate |
Cross-reactivity | rhesus monkey ( Macaca mulatta) pigtail monkey ( Macaca nemestrina) african green monkey ( Chlorocebus aethiops) chimpanzee ( Pan troglodytes) |
Antigen | CD107a (LAMP-1) |
Alternative names of antigen | LAMPA, LGP120, LAMP-1 |
Molecular mass of antigen [kDa] | 42 |
Distribution of antigen | dendritic cells, endothelial cells, epithelial cells, granulocytes, macrophages, platelets, T cells, neutrophils |
Entrez Gene ID | 3916 |
RRID | AB_2751900, AB_2783907, AB_2783906, AB_2751948, AB_2751901, AB_2751945, AB_2751898, AB_2783905, AB_2783904, AB_2751946, AB_2751899, AB_2801739, AB_2801736, AB_2819409, AB_2819408, AB_2751947 |
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