Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.