Name: CD107a (LAMP-1) Antibody, anti-human
Availability: InStock

Flow cytometry

Conjugate:	FITC
Recommended dilution:	1:50
Remark:	The antibody is suited for staining of formaldehyde-fixed cells.
Tested:	QC tested
Products used:	130-119-957, 130-119-871

MICS (MACSima Imaging Cyclic Staining)

Conjugate:	PE
Recommended dilution:	1:50
Fixation:	PFA
Tested:	during development
Products used:	130-119-958, 130-119-872
Compatible applications:	IF, IHC

Extended validation for CD107a (LAMP-1) Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with H4A3
REA792	++
508921	+
Cells were incubated with an excess of purified unconjugated CD107a (LAMP-1) (H4A3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

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Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

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Specifications for CD107a (LAMP-1) Antibody, anti-human

Overview

Clone H4A3 recognizes the CD107a antigen, also known as lysosome-associated membrane protein 1 (LAMP-1), a 110–140 kDa type I membrane glycoprotein. It is a widely expressed intracellular protein, located in the lysosomal/endosomal membrane. CD107a (LAMP-1) transiently located on the plasma membrane can be used as a marker for CD8

⁺

T cell degranulation following stimulation. It is also expressed to a lower extent on activated NK cells.

Alternative names

LAMPA, LGP120, LAMP-1

Detailed product information

Technical specifications

Clone	H4A3
Clonality	monoclonal
Isotype	mouse IgG1κ
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human, non-human primate
Cross-reactivity	rhesus monkey ( Macaca mulatta ) , pigtail monkey ( Macaca nemestrina ) , african green monkey ( Chlorocebus aethiops ) , chimpanzee ( Pan troglodytes ) , baboon
Antigen	CD107a (LAMP-1)
Alternative names of antigen	LAMPA, LGP120, LAMP-1
Molecular mass of antigen [kDa]	42
Distribution of antigen	dendritic cells, endothelial cells, epithelial cells, granulocytes, macrophages, platelets, T cells, neutrophils
Entrez Gene ID	3916
RRID	AB_2751900, AB_2783907, AB_2783906, AB_2751948, AB_2751901, AB_2751945, AB_2751898, AB_2783905, AB_2783904, AB_2751946, AB_2751899, AB_2801739, AB_2801736, AB_2819409, AB_2819408, AB_2751947

References for CD107a (LAMP-1) Antibody, anti-human

Publications

Betts, M. R. et al. (2003)
Sensitive and viable identification of antigen-specific CD8
⁺
T cells by a flow cytometric assay for degranulation.
J. Immunol. Methods 281: 65-78

Applications

Extended validation