CD105 MicroBeads were developed for the isolation of CD105
+
endothelial cells from single-cell suspensions of endothelial tissues, as well as for the isolation of mesenchymal stromal cells (MSCs) from bone marrow aspirate. CD105 MicroBeads can, for example, be used for the isolation of endothelial cells from:
  • umbilical cord veins,
  • arterial vessels1,
  • tumors2,
  • cultures of endothelial cells that are derived from synovial tissue.3

Data and images for CD105 MicroBeads, human

Figures

Figure 1

View details
Separation of CD105
+
endothelial cells from a single-cell preparation of umbilical cord vein tissue using CD105 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Figure 1

Separation of CD105
+
endothelial cells from a single-cell preparation of umbilical cord vein tissue using CD105 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Figure 2

View details
CD105
+
cells isolated from umbilical cord vein endothelium.

Figure 2

CD105
+
cells isolated from umbilical cord vein endothelium.

Figure 3

View details
Immunomagnetically selected CD105
+
cells after 25 days in culture. Cells were isolated from the bone marrow aspirate of an acute myeloid leukemia patient.
4

Figure 3

Immunomagnetically selected CD105
+
cells after 25 days in culture. Cells were isolated from the bone marrow aspirate of an acute myeloid leukemia patient.
4

Specifications for CD105 MicroBeads, human

Overview

CD105 MicroBeads were developed for the isolation of CD105
+
endothelial cells from single-cell suspensions of endothelial tissues, as well as for the isolation of mesenchymal stromal cells (MSCs) from bone marrow aspirate. CD105 MicroBeads can, for example, be used for the isolation of endothelial cells from:
  • umbilical cord veins,
  • arterial vessels1,
  • tumors2,
  • cultures of endothelial cells that are derived from synovial tissue.3

Detailed product information

Background information

The CD105 antigen (endoglin) serves as a receptor for the growth and differentiation factors TGF-β
1
and TGF-β
3
, and is expressed on mature endothelial cells, on MSCs with mesodermal differentiation capacity, and on some leukemic cells of B lymphoid and myeloid origin. The CD105 MicroBeads recognize the same molecular antigen as anti-SH2 antibodies-the most commonly employed antigen for the definition of cultivated MSCs.

Downstream applications

CD105 MicroBeads can be used for studies on mesengenesis and for
in vitro
investigations on hematological disorders.
4
CD105
+
bone marrow cells also show multipotent differentiation
in vitro5
and the capacity to form bone
in vivo
without prior cultivation or differentiation
6
.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for CD105 MicroBeads, human

Publications

  1. Dentelli, P. et al. (2007) C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium. Blood 109: 4264-4271
  2. Fonsato, V. et al. (2006) Expression of Pax2 in human renal tumor-derived endothelial cells sustains apoptosis resistance and angiogenesis. Am. J. Pathol. 168: 706-713
  3. Santiago, B. et al. (2006) CXCL12 is displayed by rheumatoid endothelial cells through its basic amino-terminal motif on heparan sulfate proteoglycans. Arthritis Res. Ther. 8: R43
  4. Campioni et al. (2003) MACS&more 7(1): 11-13
  5. Majumdar, M. K. et al. (2000) Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells. J. Cell. Physiol. 185: 98-106
  6. Aslan, H. et al. (2006)
    Osteogenic differentiation of noncultured immunoisolated bone marrow-derived CD105
    +
    cells.
    Stem Cells 24: 1728-1737

Related products for
CD105 MicroBeads, human

6 products available | view all

Seems like you are coming from USA!
Do you want to visit our website in your country?