CD10 MicroBead Kit, human

Name: CD10 MicroBead Kit, human
Availability: InStock

CD10 MicroBead Kit, human

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

⁺

cells from bone marrow or other cell sources.

Product data and images for CD10 MicroBead Kit, human

Figures

Figure 1

CD10

⁺

cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.

Unseparated fraction	After separation
View details CD10 MicroBead Kit, human Figure 1 CD10 ⁺ cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.	View details CD10 MicroBead Kit, human Figure 1 CD10 ⁺ cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.

Specifications for CD10 MicroBead Kit, human

Overview

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

⁺

cells from bone marrow or other cell sources.

Detailed product information

Background information

CD10, the human common acute lymphoblastic leukemia antigen (CALLA), is a 100 kDa cell surface molecule identical to human membrane-associated neutral endopeptidase (NEP) and also known as neprilysin or enkephalinase. Human CD10 is expressed on a wide variety of normal and neoplastic cell types from different tissues including neural and hematopoietic cells.

CD10 is expressed on pre– and pro–B cells and is involved in B cell development and differentiation. The antigen is also present on mature neutrophils, T cell precursors, some T cell leukemias/lymphomas and on neoplastic cells of several B cell leukemias/lymphomas.¹

^,

²

Applications

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

⁺

cells from bone marrow or other cell sources. It can also be used for the isolation of CD10

⁺

B cells from B cell leukemias/lymphomas.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD10 MicroBead Kit, human

Documents and Protocols

Protocols

Isolation of CD10⁺ B cells from human bone marrow or lysed blood

References for CD10 MicroBead Kit, human

Publications

Shipp, M. A. and Look, A. T. (1993) Hematopoietic differentiation antigens that are membrane-associated enzymes: cutting is the key! Blood 82: 1052-1070
Kalled, S. L. et al. (1995) The distribution of CD10 (NEP 24.11, CALLA) in human and mice is similar in non-lymphoid organs but differ within the hematopoietic system: absence on murine T and B lymphoid progenitors. Eur. J. Immunol. 25: 677-687

Data and images

Data and images for CD10 MicroBead Kit, human

Figures

Figure 1

CD10

⁺

cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.

Unseparated fraction	After separation
View details CD10 MicroBead Kit, human Figure 1 CD10 ⁺ cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.	View details CD10 MicroBead Kit, human Figure 1 CD10 ⁺ cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.

Specifications

Specifications for CD10 MicroBead Kit, human

Overview

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

⁺

cells from bone marrow or other cell sources.

Detailed product information

Background information

CD10, the human common acute lymphoblastic leukemia antigen (CALLA), is a 100 kDa cell surface molecule identical to human membrane-associated neutral endopeptidase (NEP) and also known as neprilysin or enkephalinase. Human CD10 is expressed on a wide variety of normal and neoplastic cell types from different tissues including neural and hematopoietic cells.

CD10 is expressed on pre– and pro–B cells and is involved in B cell development and differentiation. The antigen is also present on mature neutrophils, T cell precursors, some T cell leukemias/lymphomas and on neoplastic cells of several B cell leukemias/lymphomas.¹

^,

²

Applications

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

⁺

cells from bone marrow or other cell sources. It can also be used for the isolation of CD10

⁺

B cells from B cell leukemias/lymphomas.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources

Resources for CD10 MicroBead Kit, human

Documents and Protocols

Protocols

Isolation of CD10⁺ B cells from human bone marrow or lysed blood

References

References for CD10 MicroBead Kit, human

Publications

Shipp, M. A. and Look, A. T. (1993) Hematopoietic differentiation antigens that are membrane-associated enzymes: cutting is the key! Blood 82: 1052-1070
Kalled, S. L. et al. (1995) The distribution of CD10 (NEP 24.11, CALLA) in human and mice is similar in non-lymphoid organs but differ within the hematopoietic system: absence on murine T and B lymphoid progenitors. Eur. J. Immunol. 25: 677-687

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CD10 MicroBead Kit, human

CD10 MicroBead Kit, human

Product data and images for CD10 MicroBead Kit, human

Figures

Figure 1