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XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). |
Before separation | |
Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). | Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). |
After separation | |
Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). | Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). |
XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). |
Before separation | |
Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). | Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). |
After separation | |
Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). | Anti-XCR1 MicroBead Kit (Spleen), mouseFigure 1XCR1 + dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio ®Green, CD11c-VioBlue ®, Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant ® Analyzer 10. Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c +MHC class II + cells, stained for cross-presenting DC markers (CD8a and XCR1). |
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