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Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. |
Unseparated fraction | Negative fraction |
Anti-SSEA-1 (CD15) MicroBeads, human and mouseFigure 1Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. | Anti-SSEA-1 (CD15) MicroBeads, human and mouseFigure 1Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. |
Isolated mouse ES cells | |
Anti-SSEA-1 (CD15) MicroBeads, human and mouseFigure 1Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. |
Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. |
Unseparated fraction | Negative fraction |
Anti-SSEA-1 (CD15) MicroBeads, human and mouseFigure 1Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. | Anti-SSEA-1 (CD15) MicroBeads, human and mouseFigure 1Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. |
Isolated mouse ES cells | |
Anti-SSEA-1 (CD15) MicroBeads, human and mouseFigure 1Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2K k were taken either before or after MACS ® Separation and stained with anti-H-2K k-PE before analysis by flow cytometry. Expression of ectopic H-2K k was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. |
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