Anti-mPDCA-1 MicroBeads, mouse

Name: Anti-mPDCA-1 MicroBeads, mouse
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Anti-mPDCA-1 MicroBeads, mouse

Anti-mPDCA-1 MicroBeads have been developed for the enrichment of mouse plasmacytoid dendritic cells (PDCs) from lymphoid tissues.

Product data and images for Anti-mPDCA-1 MicroBeads, mouse

Figures

Figure 1

Mouse mPDCA-1

⁺

PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Spleen cells before separation	Spleen cells depleted of mPDCA-1 ⁺ PDCs
View details Anti-mPDCA-1 MicroBeads, mouse Figure 1 Mouse mPDCA-1 ⁺ PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Anti-mPDCA-1 MicroBeads, mouse Figure 1 Mouse mPDCA-1 ⁺ PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Enriched mPDCA-1 ⁺ PDCs
View details Anti-mPDCA-1 MicroBeads, mouse Figure 1 Mouse mPDCA-1 ⁺ PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-mPDCA-1 MicroBeads, mouse

Overview

Anti-mPDCA-1 MicroBeads have been developed for the enrichment of mouse plasmacytoid dendritic cells (PDCs) from lymphoid tissues.

Detailed product information

Background information

The mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1) is specifically expressed on mouse PDCs, a subset of CD11c

⁺

dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. In mouse spleen, bone marrow, and lymph nodes, mPDCA-1 is exclusively expressed on cells which are CD11c

^low

, CD45R (B220)

⁺

,Ly-6C

^high

, MHC Class II

^low

, CD8a

^low/-

, CD80

^low/-

, CD86

^-

, CD40

^-

, CD11b

^-

, CD90

^-

, CD49b (DX5)

^-

, CD3

^-

, CD19

^-

, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed, that all CD11c

⁺

CD45R (B220)

⁺

Ly-6C

⁺

PDCs are mPDCA-1

⁺

and that mPDCA-1 expression is restricted to PDCs in non-treated mice.

Downstream applications

The isolated PDCs are suitable for studies on their functional properties, e.g. antigen uptake and presentation, crosspriming of cytotoxic T cells, T cell activation or tolerance induction, or T helper cell polarization by PDCs in experimental mouse models of infection and autoimmunity.

Highly pure PDCs were isolated with Anti-mPDCA-1 MicroBeads and have been further analyzed for IDO-dependent tolerance-induction.

¹

Anti-mPDCA-1 MicroBeads have also been used to isolate PDCs for subsequent analysis of EBV-induced gene 3 (EBI3) expression and regulation in mouse DC subsets.

²

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

References for Anti-mPDCA-1 MicroBeads, mouse

Publications

Fallarino, F. et al. (2005) Ligand and cytokine dependence of the immunosuppressive pathway of tryptophan catabolism in plasmacytoid dendritic cells. Int. Immunol. 17: 1429-1438
Wirtz, S. et al. (2005) EBV-induced gene 3 transcription is induced by TLR signaling in primary dendritic cells via NF-kappa B activation. J Immunol 174: 2814-2824

Data and images