Positive selection or depletion of cells expressing human LGR5 antigen, such as colon cancer stem cells, or stem cells from the small intestine, colon, hair follicle, etc.

Data and images for Anti-LGR5 MicroBeads, human

Figures

Figure 1

The LGR5
+
subpopulation of human colorectal adenocarcinoma cells (LoVo) was isolated using Anti-LGR5 MicroBeads, an LS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with Labeling Check Reagent-APC (# 130-098-892) and CD44-PE (# 130-098-108) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
LGR5
+
cells
View details

Figure 1

The LGR5
+
subpopulation of human colorectal adenocarcinoma cells (LoVo) was isolated using Anti-LGR5 MicroBeads, an LS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with Labeling Check Reagent-APC (# 130-098-892) and CD44-PE (# 130-098-108) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

The LGR5
+
subpopulation of human colorectal adenocarcinoma cells (LoVo) was isolated using Anti-LGR5 MicroBeads, an LS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with Labeling Check Reagent-APC (# 130-098-892) and CD44-PE (# 130-098-108) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-LGR5 MicroBeads, human

Overview

Positive selection or depletion of cells expressing human LGR5 antigen, such as colon cancer stem cells, or stem cells from the small intestine, colon, hair follicle, etc.

Detailed product information

Background information

LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression.
LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells
1,2
. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development
3,4,5,6
, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5
+
crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression
7,8
.

Columns

For positive selection: LS, XS, or autoMACS
®
Columns. For depletion: LD, CS, D, or autoMACS Columns.
Note: This product is not suitable for cell separation with an MS Column.

Resources for Anti-LGR5 MicroBeads, human

References for Anti-LGR5 MicroBeads, human

Publications

  1. Dame. M.K. et al. (2018) Identification, isolation and characterization of human LGR5-positive colon adenoma cells Development 145(6 (dev153049)): doi: 10.1242
  2. de Lau, W. et al. (2011) Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476(7360): 293-297
  3. Carmon, K. S. et al. (2011) R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/β-catenin signaling. Proc. Natl. Acad. Sci. U.S.A. 108: 11452-11457
  4. Barker, N. et al. (2007) Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449(7165): 1003-1007
  5. Jaks, V. et al. (2008) Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat. Genet. 40: 1291-1299
  6. Barker, N. et al. (2010)
    Lgr5
    +
    stem cells drive self-renewal in the stomach and build long-lived gastric units
    in vitro
    .
    Cell Stem Cell 6(1): 25-36
  7. Barker, N. et al. (2012)
    LGR5
    +
    stem/progenitor cells contribute to nephron formation during kidney development.
    Cell Rep 2: 540-552
  8. Barker, N. et al. (2009) Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457: 608-611
  9. Kemper, K. et al. (2012) Monoclonal antibodies against lgr5 identify human colorectal cancer stem cells. Stem Cells 30(11): 2378-2386

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