The Anti-GLAST (ACSA-1) MicroBead Kit has been developed for the separation of astrocytes based on the expression of GLAST.

Data and images for Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse, rat

Figures

Figure 1

GLAST
+
cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Mouse forebrain cells before separation
GLAST (ACSA-1)-negative cells
View details

Figure 1

GLAST
+
cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

GLAST
+
cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
GLAST (ACSA-1)
-positive cells
View details

Figure 1

GLAST
+
cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse, rat

Overview

The Anti-GLAST (ACSA-1) MicroBead Kit has been developed for the separation of astrocytes based on the expression of GLAST.

Detailed product information

Background information

The antibody ACSA-1 (astrocyte cell surface antigen-1) is specific for an extracellular epitope of the astrocyte specific transmembrane glycoprotein GLAST in the central nervous system (CNS).
1,2
Besides GLT-1, GLAST is the most abundant glutamate transporter and is predominantly expressed by astrocytes in the developing and neonatal mammalian CNS. Also radial glia, which belong to the astrocyte lineage and play important roles in development, are known to express GLAST. Postnatally, radial glia only persist in a few regions, such as Bermann glia in the cerebellum, Müller glia in the retina, and radial glia in the dentate gyrus of the adult hippocampus.
3,4

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

Resources for Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse, rat

References for Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse, rat

Publications

  1. Storck, T. et al. (1992)
    Structure, expression, and functional analysis of a Na
    +
    -dependent glutamate/aspartate transporter from rat brain.
    Proc. Natl. Acad. Sci. U.S.A. 8(22): 10955-10959
  2. Wahle, S. and Stoffel, W. (1996) Membrane topology of the high-affinity L-glutamate transporter (GLAST- 1) of the central nervous system. J. Cell Biol. 135(6): 1867-1877
  3. Kimelberg, H.K. (2004) The problem of astrocyte identity. Neurochem. Int. 45(2-3): 191-202
  4. Kriegstein, A. R. and Götz, M. (2003) Radial glia diversity: a matter of cell fate. Glia 43: 37-43

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