Name: Anti-AN2 MicroBeads, human and mouse
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Product data and images for Anti-AN2 MicroBeads, human and mouse

Figures

Figure 1

AN2

⁺

cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	AN2 ^– cells
View details Figure 1 AN2 ⁺ cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Figure 1 AN2 ⁺ cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
AN2 ⁺ cells
View details Figure 1 AN2 ⁺ cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-AN2 MicroBeads, human and mouse

Overview

Anti-AN2 MicroBeads have been developed for positive selection or depletion of murine or human AN2

⁺

glial cells.

Detailed product information

Background information

AN2

is the mouse homolog of the rat NG2 and human MCSP protein. NG2 is a chondroitin sulfate glycoprotein that is expressed by glial progenitor cells in the developing and adult central nervous system (CNS) and by immature Schwann cells.

NG2/AN2

⁺

glia has been identified as a fourth major glial cell type in the mammalian CNS that is distinct from other cell types

. NG2/AN2

⁺

cells are precursors of oligodendrocytes and some protoplasmic astrocytes in the grey matter

. NG2/AN2 expression is down-regulated before the cells undergo terminal differentiation. However, a population of NG2/AN2

⁺

cells remain in the white and grey matter after oligodendrocytes are generated

^4-6

NG2/AN2

⁺

glial cells participate in neuron-glial circuits by receiving presynaptic input from neurons and responding to neurotransmitters released by synapses

⁷

Many highly migratory neural tumors including melanomas express NG2/AN2.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Resources for Anti-AN2 MicroBeads, human and mouse

Documents and Protocols

Brochures/posters

Neuroscience research

References for Anti-AN2 MicroBeads, human and mouse

Publications

Trotter, J. et al. (2010) NG2 cells: Properties, progeny and origin. Brain Res. Rev. 63: 72-82
Zhu, X. et al. (2008) NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 132: 145-157
Zhu, X. et al. (2011) Age-dependent fate and lineage restriction of single NG2 cells. Development 138: 745-753
Diers-Fenger, M. et al. (2001) AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: isolation, differentiation, and association with radial glia. Glia 34: 213-228
Wigley, R. et al. (2007) Morphological and physiological interactions of NG2-glia with astrocytes and neurons. J. Anat. 210: 661-670
Stegmuller, J. et al. (2002) AN2, the mouse homologue of NG2, is a surface antigen on glial precursor cells implicated in control of cell migration. J. Neurocytol. 31: 497-505
Peters, A. (2004) A fourth type of neuroglial cell in the adult central nervous system. J. Neurocytol. 33: 345-357
Nishiyama, A. et al. (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat. Rev. Neurosci. 10: 9-22

Data and images

Data and images for Anti-AN2 MicroBeads, human and mouse

Figures

Figure 1

AN2

⁺

Before separation	AN2 ^– cells
View details Figure 1 AN2 ⁺ cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Figure 1 AN2 ⁺ cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
AN2 ⁺ cells
View details Figure 1 AN2 ⁺ cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for Anti-AN2 MicroBeads, human and mouse

Overview

Anti-AN2 MicroBeads have been developed for positive selection or depletion of murine or human AN2

⁺

glial cells.

Detailed product information

Background information

AN2

NG2/AN2

⁺

glia has been identified as a fourth major glial cell type in the mammalian CNS that is distinct from other cell types

. NG2/AN2

⁺

cells are precursors of oligodendrocytes and some protoplasmic astrocytes in the grey matter

. NG2/AN2 expression is down-regulated before the cells undergo terminal differentiation. However, a population of NG2/AN2

⁺

cells remain in the white and grey matter after oligodendrocytes are generated

^4-6

NG2/AN2

⁺

glial cells participate in neuron-glial circuits by receiving presynaptic input from neurons and responding to neurotransmitters released by synapses

⁷

Many highly migratory neural tumors including melanomas express NG2/AN2.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Resources

Resources for Anti-AN2 MicroBeads, human and mouse

Documents and Protocols

Brochures/posters

Neuroscience research

References

References for Anti-AN2 MicroBeads, human and mouse

Publications

Trotter, J. et al. (2010) NG2 cells: Properties, progeny and origin. Brain Res. Rev. 63: 72-82
Zhu, X. et al. (2008) NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 132: 145-157
Zhu, X. et al. (2011) Age-dependent fate and lineage restriction of single NG2 cells. Development 138: 745-753
Diers-Fenger, M. et al. (2001) AN2/NG2 protein-expressing glial progenitor cells in the murine CNS: isolation, differentiation, and association with radial glia. Glia 34: 213-228
Wigley, R. et al. (2007) Morphological and physiological interactions of NG2-glia with astrocytes and neurons. J. Anat. 210: 661-670
Stegmuller, J. et al. (2002) AN2, the mouse homologue of NG2, is a surface antigen on glial precursor cells implicated in control of cell migration. J. Neurocytol. 31: 497-505
Peters, A. (2004) A fourth type of neuroglial cell in the adult central nervous system. J. Neurocytol. 33: 345-357
Nishiyama, A. et al. (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat. Rev. Neurosci. 10: 9-22

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Anti-AN2 MicroBeads, human and mouse

Anti-AN2 MicroBeads, human and mouse

Product overview

Product data and images for Anti-AN2 MicroBeads, human and mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for Anti-AN2 MicroBeads, human and mouse

Overview

Detailed product information

Background information

Columns

Resources for Anti-AN2 MicroBeads, human and mouse

Documents and Protocols

References for Anti-AN2 MicroBeads, human and mouse

Publications

Data and images for Anti-AN2 MicroBeads, human and mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for Anti-AN2 MicroBeads, human and mouse

Overview

Detailed product information

Background information

Columns

Resources for Anti-AN2 MicroBeads, human and mouse

Documents and Protocols

References for Anti-AN2 MicroBeads, human and mouse

Publications

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FcR Blocking Reagent, mouse

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Pre-Separation Filters (70 µm)

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gentleMACS Dissociator

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