The Anti-ABCG2 (CD338) MicroBeads have been developed for the positive selection of ABCG2
+
(CD338) cells, also referred as Side Population cells (SP), in a single step. SP cells can be isolated from various sources such as bone marrow or cord blood.

Data and images for Anti-ABCG2 (CD338) MicroBeads, human

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The ABCG2
+
subpopulation of human breast cancer cells (MCF7)was isolated using Anti‑ABCG2 (CD338) MicroBeads, an LS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with Labeling Check Reagent‑PE (# 130‑095‑228) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
The ABCG2
+
subpopulation of human breast cancer cells (MCF7)was isolated using Anti‑ABCG2 (CD338) MicroBeads, an LS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with Labeling Check Reagent‑PE (# 130‑095‑228) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-ABCG2 (CD338) MicroBeads, human

Overview

The Anti-ABCG2 (CD338) MicroBeads have been developed for the positive selection of ABCG2
+
(CD338) cells, also referred as Side Population cells (SP), in a single step. SP cells can be isolated from various sources such as bone marrow or cord blood.

Detailed product information

Background information

ABCG2, also known as CD338 or BCRP1, is a multi-drug resistance (MDR) family protein that is a member of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extracellular and intracellular membranes. It functionsas a xenobiotic transporter which may play a role in multi-drug resistance to chemotherapeutic agents1. ABCG2 is highly expressed on primitive side population (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342 by ABCG2. It was shown that expression of the ABCG2 gene is a conserved feature of stem cells from a wide variety of sources.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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