Clone:
REA402
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, IF, IHC
Alternative names:
ACTN2, CMD1AA, CMH23

Specifications for α-Actinin (Sarcomeric) Antibody, anti-human/mouse/rat, REAfinity™

Overview

Clone REA402 recognizes the sarcomeric α-actinin antigen, a 100 kDa actin-binding protein, which occupies a strategic role in the assembly and maintenance of stress fibers of non-muscle cells and the myofibrils of muscle cells. α-actinins have been highly conserved throughout evolution and are largely collinear proteins that share three conserved functional domains. Functionally, α-actinins form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. Two isoforms of α-actinin have been identified, a muscle-specific sarcomeric isoform and a non-sarcomeric isoform. The major – and best characterized – functional difference among α-actinin isoforms is calcium sensitivity for actin-binding. The binding of α-actinin to actin by non-muscle cytoskeletal isoforms is dependent on the calcium concentration, whereas this interaction is independent of calcium concentration in sarcomeric striated and smooth muscle isoforms. Studies on skeletal and cardiac cells have localized sarcomeric α-actinin to Z-bands in striated myofibrils, to precursor I-Z-I-like complexes in muscle undergoing myofibrillogenesis, and to vinculin-positive adhesion plaques and adherens junctions.
Additional information: Clone REA402 displays negligible binding to Fc receptors.

Alternative names

ACTN2, CMD1AA, CMH23

Detailed product information

Technical specifications

CloneREA402
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, rat, non-human primate, other
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
, sheep, rabbit, lizard, feline, canine, snake, fish, goat, chicken, bovine, frog, pig, hamster
Antigenα-Actinin (Sarcomeric)
Alternative names of antigenACTN2, CMD1AA, CMH23
Molecular mass of antigen [kDa]104
Distribution of antigenstem cells, ES and iPS cells
Entrez Gene ID88
RRIDAB_2751827, AB_2857660, AB_2857635, AB_2819563, AB_2819533, AB_2654310, AB_2654311, AB_2654312, AB_2751858

References for α-Actinin (Sarcomeric) Antibody, anti-human/mouse/rat, REAfinity™

Publications

  1. Young, P. et al. (1998) Molecular structure of the sarcomeric Z-disk: two types of titin interactions lead to an asymmetrical sorting of alpha-actinin. EMBO J. 17(6): 1614-1624
  2. Zhang, Z. Q. et al. (2009) Sarcomeric-alpha-actinin defective in vinculin-binding causes Z-line expansion and nemaline-like body formation in cultured chick myotubes. Exp. Cell Res. 315(5): 748-759
  3. Lu, M. H. et al. (1992) The vinculin/sarcomeric-alpha-actinin/alpha-actin nexus in cultured cardiac myocytes. J. Cell Biol. 117(5): 1007-1022

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