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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Viral or fungal infections are a major cause of morbidity and mortality in the period of immune recovery after hematopoietic stem cell transplantation (HSCT)1,2. Adoptively transferred antigen-specific T cells have been shown to restore protective immunity and control established adenovirus (AdV)3,4,13, cytomegalovirus (CMV)5,6, and Epstein-Barr virus (EBV)7,8 infections after HSCT in adults as well as in children.
However, the manufacturing of those rare antigen-specific T cells under GMP-compliant conditions is lengthy and very labor-intensive. The CliniMACS® Cytokine Capture System (CCS) (IFN-gamma) addresses all these challenges and provides the perfect solution.
Utilizing the CliniMACS Prodigy® Platform, this method allows the fast and fully automated generation of viable antigen-specific CD4+ and CD8+ T cells, e.g., with specificity for HCMV9–12, EBV14, AdV14, BKV15 or multivirus-specificity14. The whole process happens in a closed sterile system and only takes approximately 12 hours.
The first step of the fully automated CliniMACS Prodigy® manufacturing process is the stimulation of PBMCs with the antigen of interest, e.g. with MACS® GMP HCMV PepTivator® pp65 (CMV). The automated antigen-specific stimulation of T cells is performed with high quality and GMP-compliant reagents.
Stimulation with MACS® GMP PepTivator® Peptide Pools
MACS GMP PepTivator Peptide Pools are overlapping oligopeptides covering the complete sequence of a respective antigen. They provide the following benefits:
T cell culture with TexMACS™ GMP Medium
TexMACS GMP Medium is an optimized T cell culture medium.
During the automated CliniMACS Prodigy process, the target cells are enriched by a 2-step labeling approach.
The CliniMACS Catchmatrix Reagent and the CliniMACS Enrichment Reagent are components of the CliniMACS Cytokine Capture System (IFN-gamma).
For the manufacture and use of the antigen-specific T cells, national and international legislation and regulations must be followed.
Miltenyi Biotec as the provider of the CliniMACS CCS-IFN System does not give any recommendation regarding the use of the manufactured cells for therapeutic purposes and does not make any claim regarding a clinical benefit.
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