Protocols to improve your CAR T cell workflow.
For the automated T cell transduction process on the CliniMACS Prodigy®, please refer to GMP-engineered T cells.
Tips and tricks to get your CAR T cell research moving.
Introduction into Miltenyi Biotec’s workflow for CAR T cell manufacturing for research use, as well as possibilities to easily translate the workflow into the clinic.
Following on from our renowned webinar “Improve your CAR T cell research”, this webinar will cover our latest product solutions and strategies to efficiently move your CAR T cell research forward. Tune in and learn how to: Isolate fully functional T cells directly from whole blood and blood products, boost lentiviral T cell transduction efficiency, easily activate and expand your T cells without hassle, and advance your IPC and QC with our latest flow analysis solutions.
Starting your workflow with a well-defined, enriched T cell population is key to obtain robust, standardized, and reproducible results. Depending on the starting material, PBMCs or blood products, you choose either standard MACS® MicroBeads and Isolation Kits or the innovative StraightFrom® Technology. But independent of the approach you decide for, the basis is always the proven and most cited MACS MicroBead Technology. In addition, to enable a seamless transition of your research workflow to the CliniMACS Prodigy® Platform, our MACS MicroBeads are available in MACS GMP Quality.
Positive selection enables straightforward isolation of CD4+ and CD8+ T cells from PBMCs using MACS MicroBeads and MicroBead Kits.
If subsequent magnetic sorting steps are required, choose untouched cell isolation: MACS T Cell Isolation Kits allow for isolation of unlabeled target cells via depletion of non-target cells.
Increase throughput and productivity with StraightFrom® Technology for T cell isolation directly from whole blood and blood products (buffy coat, LRSC, and Leukopak®) without density gradient centrifugation or RBC lysis.
The MACSprep™ CD19 CAR MicroBead Kit has been developed for fast and convenient CAR T cell isolation from fresh blood samples.
Physiological T cell activation is vital to start the proliferation and expansion process and facilitate an efficient T cell transduction. Optimal T cell expansion relies on the synergistic effect of T Cell TransAct™, TexMACS™ Medium, and MACS Cytokines.
Transgene expression depends on gene transfer efficiency. However, viral transduction of suspension cells with retroviral vectors is often inefficient and requires the use of transduction enhancers.
Using the synthetic, non-toxic soluble Vectofusin-1® with a simple and easy-to-follow protocol enhances the transduction efficiency of viral vectors.
As the success of CAR T cell therapies has been associated with the phenotype, activation status, and functional profiling of CAR T cells, the reliable and standardized characterization of those parameter is crucial.
Our CAR T cell analytics portfolio provides all you need for reliable, reproducible, and standardized analysis. Use our high-quality reagents, instruments, and software to streamline your CAR T cell analysis – from basic research and discovery to IPC/QC in clinical manufacturing.
Tune into the recordings of Miltenyi Biotec´s Cell Therapy Analytics Day 2021 to discover lessons learned, master best practices, and overcome regulatory hurdles in order to simplify your cell manufacturing analytics
Standardizing flow cytometry assays in cell manufacturing
Watch our three-part webinar series on automated tools and learn how to take your standardization efforts to the next level.
Our optimized and validated panels of fluorochrome-conjugated REAfinity™ Recombinant Antibodies and CAR Detection Reagents help to streamline the phenotypic characterization of CAR T cells. For maximum reproducibility and standardization of in-process-control (IPC) and quality control (QC) for your clinical manufacturing process, combine the StainExpress™ Dried Antibody Cocktails with the MACSQuant® Analyzer and the Express Modes.
Validated panels for CAR T cell flow analysis
Benefit from Miltenyi Biotec Tested Panels (MBTP) including antibody panels, step-by-step protocols, and gating strategies to accelerate your CAR T cell flow analysis.
Our ready-to-use biotinylated CAR Detection Reagents have been developed for the detection of T cells that are engineered to express a specific CAR on the cell surface, e.g., CD19 CAR or CD22 CAR.
We offer flow cytometry solutions to improve the quality of potency assay results. They can, for example, be used for high-throughput and automated in vitro potency assays to assess CAR T cells’ antigen-specific killing, T cell phenotype, intracellular cytokine detection, as well as effector cytokine secretion.
Rapid Cytokine Inspector Kits offer the fastest combination of surface marker and intracellular cytokine staining
Conventional staining to detect effector cytokine secreting CAR T cells
MACSPlex Cytokine Kits for quatification of up to 12 analytes in one supernatant
Ultra high content imaging allows for CAR T cell target discovery, in-depth tumor infiltration analysis, preclinical CAR T cell target safety as well as CAR T cell biodistribution.