In this application protocol, we describe how to isolate untouched human tumor cells in an easy workflow that improves sensitivity and reduces bias in downstream applications, such as next-generation sequencing and tumor cell culture. Solid tumors are dissociated into viable single-cell suspensions and untouched tumor cells are then isolated using MACS® technology.
Create a viable single-cell suspension from a solid human tumor using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, human. Follow the protocol from the kit data sheet.
Download data sheet
Isolate untouched human tumor cells using the Tumor Cell Isolation Kit, human and LS Columns. Follow the protocol of the kit data sheet.
Download the data sheet
Tumor Cell Isolation Kit, human
We recommend filtering the magnetically labeled cell suspension to guarantee it is single-celled before separating it on the column.
The following is a list of additional resources that may be of interest:
The materials and methods described in this Application are for the manual protocol. Automated cell isolation can be performed with the autoMACS® Pro or the MultiMACS™ Cell24 instruments.
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