This application protocol describes step-by-step the immunostaining and clearing of primary human tumors and patient-derived xenografts using the MACS® Clearing Kit for downstream 3D imaging analysis. The MACS® Clearing Kit enables the immunostaining and clearing of up to 20 tumor samples of approximately 5×5×5 mm3.
For more information about light sheet fluorescence microscopy refer to Light sheet microscopy.
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