In this application protocol, we describe an easy and convenient technique using MACSxpress® Technology for the efficient purification of untouched immune cells from human whole blood in 20 minutes. Up to 30 mL of whole blood can be processed in a single tube, enabling the fast isolation of a large number of NK cells for downstream applications. The procedure does not require density gradient centrifugation, which minimizes pipetting steps and aerosol formation for a high level of safety.
Isolate NK cells from up to 30 mL whole blood using the MACSxpress® Whole Blood NK Cell Isolation Kit, human. The kit enables isolation directly from blood in a single tube and in 20 minutes, without the need for density gradient centrifugation. Aggregated erythrocytes and platelets sediment, while magnetically labeled non-target cells are retained in the magnetic field of the MACSxpress Separator. The supernatant containing the target cells is then collected.
Follow the protocol of the kit data sheet.
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For NK cell expansion, PBMCs or isolated NK cells are cultured for up to 20 days in the presence of Anti-Biotin MACSiBead™ Particles loaded with CD2 and CD335 (NKp46) antibodies from the NK Cell Activation/Expansion Kit, human. Follow the protocol of the kit data sheet.
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