Fully automated light sheet microscope for 3D imaging of multiple samples ...

UltraMicroscope Blaze™

Automated light sheet imaging of large samples

Benefits Features and specifications Applications Support

Benefits

Full automation

The UltraMicroscope Blaze enables an automated sample release and smooth switching between different objectives and magnification lenses, while keeping images sharp with the autofocus feature.

Large sample chamber

Benefit from the large sample chamber that can contain a whole cleared mouse model or multiple samples at once, which can then be imaged sequentially together.

Easy adaptation

Whether imaging large organs or even entire mouse models, our wide range of dipping caps guarantees high image quality in all clearing media - from water to organic solvents.

High-resolution

The combination of the MI Plan objective lenses and the cutting-edge light sheet illumination technology ensures unprecedented image quality from overview to sub-cellular details.

Features and specifications

MI Plan objectives

MI Plan objectives (1.1×, 4×, and 12×) are optimized for high-resolution 3D imaging of large samples in any clearing media.

Large automated chamber

Image a whole cleared mouse or multiple organs without user intervention and exchange samples easily by automated sample release.

Automated detection unit

Fully automated switching of all lenses combined with the autofocus feature ensure a convenient imaging process.

Cutting-edge light sheet optics

Up to six light sheets make for improved optical sectioning and homogeneous illumination.

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MI Plan objectives

MI Plan objectives (1.1×, 4×, and 12×) are optimized for high-resolution 3D imaging of large samples in any clearing media.

Large automated
chamber

Image a whole cleared mouse or multiple organs without user intervention and exchange samples easily by automated sample release.

Automated detection unit

Fully automated switching of all lenses combined with the autofocus feature ensure a convenient imaging process.

Cutting-edge light sheet optics

Up to six light sheets make for improved optical sectioning and homogeneous illumination.

Technical features

Light sheet technology tailored to your sample

View details

As the generated light sheet diverges, it approximates a plane only over a given horizontal range. This is where the light sheet is thinnest and where fluorescence detection takes place. To achieve an appropriate illumination for a particular sample, the planar range of the light sheet has to be matched with the sample size and the desired field of view (FOV). The light sheet of the UltraMicroscope Blaze is tailored to the sample size by using adjustable light sheet parameters.

A low numerical aperture (NA) of illumination results in a broad FOV at the expense of a low Z-resolution when imaging large samples (A). In contrast, a high NA results in a high Z-resolution and a narrow FOV suitable for imaging small samples (B), with a full range of gradations in between. Where both high Z-resolution and a large FOV are essential, for example when imaging larger samples, the UltraMicroscope Blaze offers dynamic horizontal focusing. To that end, a sequential series of high-resolution images are taken across the desired FOV and automatically stitched together into a single high-quality image by our ImSpector image acquisition software (C).

MI Plan objectives optimized for high-resolution light sheet imaging

View details

The UltraMicroscope Blaze with its AO configuration can host up to three MI Plan objective lenses. Generally, objective lenses can produce artifacts that increase in severity with the size of FOV. However, the flat-field correction in the MI Plan objectives generate images that are in focus throughout the area of interest. The MI Plan objective series is designed and optimized for high-resolution light sheet imaging. The combination of flat-field correction and long working distances makes these objectives well suited for imaging large samples. They can be coupled to a wide variety of dipping caps that allow high-resolution and distortion-free imaging in all clearing solutions, whether based on aqueous or organic solvents (refractive indices between 1.33 and 1.56).

Multiple light sheets optimized for uniform illumination

View details

The cutting-edge illumination optics of the UltraMicroscope Blaze generates multiple light sheets that overlap in the desired FOV and excite the sample from both sides. This results in a uniform illumination profile, improved optical sectioning, and thereby a higher image quality.

Compatibility with all imaging solutions

View details

Most clearing reagents differ from protocol to protocol, and so do their optical properties. Light can travel differently in different imaging media, which can create distortions and thereby lower the image quality. To compensate for this, the UltraMicroscope Blaze’s onboard software allows users to choose between various predefined clearing and imaging solutions. This provides the perfect setting for every imaging media.

UltraMicroscope Blaze specifications

The UltraMicroscope Blaze comes in two configurations: the zoom configuration for easy handling in a multi-user environment, or the fully automated objective (AO) configuration for high-resolution light sheet imaging.

UltraMicroscope Blaze specifications

Sheet optics
Illumination	Uni- and bi-directional
Number of light sheet	1–6
Thickness	4–24 µm
Width	1–20 mm
Numerical aperture	0.0135–0.135
Focus positioning	Dynamic
Detection optics	Zoom configuration	AO configuration
Objective lenses	2×	1.1×	4×	12×
Zoom \| magnification changer	1.26–12.6×	0.66–2.75×	2.4–10×	7.2–30×
Resolution at detector	1–10.4 µm	4.8–19.6 µm	1.3–5.4 µm	0.44–1.8 µm
Numerical aperture	0.5	0.1	0.35	0.53
Working distance	>5.7 mm	≤17 mm	≤16 mm	≤10.9 mm
FOV diagonal (5.5 MP camera)	1.7–17.3 mm	7.9–33 mm	2.2–9.1 mm	0.73–3 mm
Emission filters	Seven filters ⌀ 32 mm	Seven filters ⌀ 43 mm
Chromatic correction	Dynamic 400–850 nm
Image chamber
Imaging solution	Aqueous buffers and organic solvents
Sample travel range (x, y, z)	12.5 mm, 43.7 mm, 25 mm
Chamber size	129 mm, 51 mm, 64 mm
Multiple sample capacity	Up to five samples at once
Refractive index compatibility	1.33–1.56
General information
Dimensions (w × h × d)	67 cm × 91 cm ×52.5 cm
Weight	98 kg (w/o controller and laser)
Light sources
Laser beam combiner	Max. 5 laser lines (405, 488, 561, 639, 785 nm)*, 50 mW–100 mW per diode
Supercontinuum white light laser	460–800 nm, 1–3 mW/nm

^{*Five out of eleven available laser can be chosen for the Beam Combiner.}

Camera specifications

Detector	4.2 Megapixel sCMOS camera	5.5 Megapixel sCMOS camera
Active pixels (w × h)	2048 × 2048	2560 × 2560
Pixel size	6.5 µm × 6.5 µm	6.5 µm × 6.5 µm
Sensor size	13.3 mm × 13.3 mm; 18.8 mm diagonal	16.6 mm × 14 mm; 21.8 mm diagonal
Readout noise	0.8 med e-	1 med e-
Maximal frame rate	100 fps	100 fps
Maximum quantum efficiency	82%	60%

Resources

Selected references

Pan, C. et al. (2019) Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body. Cell 179(7): 1661-1676
Zhao, S. et al. (2020) Cellular and Molecular Probing of Intact Human Organs. Cell 180(4): 796-812

Brochures and posters

UltraMicroscope Blaze - The automation of light sheet microscopy

Applications

Video

Immuno-oncology

Pancreatic carcinoma cell line with infiltrating CAR T cells

Video

Neuroscience

Regeneration potential of neurons and axonal path finding

Cancer research

Visualization of single disseminated tumor cells in whole mouse body

Support

Service where you need it

Instrument training at MACS Academy Miltenyi Biotec facilities
Comprehensive service options provided by our globally distributed field service teams

UltraMicroscope Blaze

Automated light sheet imaging of largest samples

Order no. 150-001-760

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UltraMicroscope Blaze™

Automated light sheet imaging of large samples

Benefits

Full automation

Large sample chamber

Easy adaptation

High-resolution

Features and specifications

MI Plan objectives

Large automated chamber

Automated detection unit

Cutting-edge light sheet optics

MI Plan objectives

Large automated
chamber

Automated detection unit

Cutting-edge light sheet optics

Technical features

Light sheet technology tailored to your sample

MI Plan objectives optimized for high-resolution light sheet imaging

Multiple light sheets optimized for uniform illumination

Compatibility with all imaging solutions

UltraMicroscope Blaze specifications

Resources

Selected references

Brochures and posters

Applications

Video

Immuno-oncology

Video

Neuroscience

Cancer research

Support

Service where you need it

UltraMicroscope Blaze

Shop

Careers

About Miltenyi Biotec

Contact

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UltraMicroscope Blaze™

Automated light sheet imaging of large samples

Benefits

Full automation

Large sample chamber

Easy adaptation

High-resolution

Features and specifications

MI Plan objectives

Large automated chamber

Automated detection unit

Cutting-edge light sheet optics

MI Plan objectives

Large automatedchamber

Automated detection unit

Cutting-edge light sheet optics

Technical features

Light sheet technology tailored to your sample

MI Plan objectives optimized for high-resolution light sheet imaging

Multiple light sheets optimized for uniform illumination

Compatibility with all imaging solutions

UltraMicroscope Blaze specifications

Resources

Selected references

Brochures and posters

Applications

Video

Immuno-oncology

Video

Neuroscience

Cancer research

Support

Service where you need it

UltraMicroscope Blaze

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Large automated
chamber

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UltraMicroscope Blaze