Tumor Cell Isolation Kit, human

Name: Tumor Cell Isolation Kit, human
Availability: InStock

Tumor Cell Isolation Kit, human

The Tumor Cell Isolation Kit, human enables the enrichment of human tumor cells from primary specimens using MACS

^®

Cell Separation technology.

While depleting tumor associated cells such as lymphocyte subpopulations, fibroblasts, and endothelial cells in a fast and easy protocol, the tumor cells remain untouched, improving any downstream analysis.

Product data and images for Tumor Cell Isolation Kit, human

Figures

Figure 1

EpCAM positive human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator. Subsequently, the unseparated fraction and the negative fraction were cultured for seven days in expansion medium (90% RPMI-1640, 2mM L-Glutamine, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for vimentin (red), labeling fibroblasts, and EpCAM (green), labeling tumor cells. Cell nuclei were stained with DAPI (blue).

Before separation

Isolated human tumor cells

Figure 1

EpCAM positive human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator. Subsequently, the unseparated fraction and the negative fraction were cultured for seven days in expansion medium (90% RPMI-1640, 2mM L-Glutamine, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for vimentin (red), labeling fibroblasts, and EpCAM (green), labeling tumor cells. Cell nuclei were stained with DAPI (blue).

Figure 1

EpCAM positive human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator. Subsequently, the unseparated fraction and the negative fraction were cultured for seven days in expansion medium (90% RPMI-1640, 2mM L-Glutamine, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for vimentin (red), labeling fibroblasts, and EpCAM (green), labeling tumor cells. Cell nuclei were stained with DAPI (blue).

Figure 2

EpCAM

⁺

human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and the gentleMACS™ Octo Dissociator. The cells were fluorescently stained with CD326 (EpCAM)‑VioBlue

^®

and CD31‑PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

Isolated human tumor cells

Figure 2

EpCAM

⁺

human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and the gentleMACS™ Octo Dissociator. The cells were fluorescently stained with CD326 (EpCAM)‑VioBlue

^®

and CD31‑PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

EpCAM

⁺

human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and the gentleMACS™ Octo Dissociator. The cells were fluorescently stained with CD326 (EpCAM)‑VioBlue

^®

and CD31‑PE and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Tumor Cell Isolation Kit, human

Overview

The Tumor Cell Isolation Kit, human enables the enrichment of human tumor cells from primary specimens using MACS

^®

Cell Separation technology.

While depleting tumor associated cells such as lymphocyte subpopulations, fibroblasts, and endothelial cells in a fast and easy protocol, the tumor cells remain untouched, improving any downstream analysis.

Detailed product information

Background information

The Tumor Cell Isolation Kit, human has been designed for the enrichment of untouched human tumor cells from primary specimens. During the growth phase in vivo, tumor tissue is vascularized and infiltrated by cells of non-tumor origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells.

The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, and affected organ site. However, even when these factors are constant, the amount and composition of infiltrating cells are highly variable, which makes accurate molecular downstream analyses difficult.

The contaminating non-tumor cells lead to hybridization of non-tumor cell derived mRNA molecules to probes on microarrays and a significant reduction of sensitivity caused by measurement of irrelevant signals during next-generation sequencing or proteome analysis. In addition, the culture of human tumor cells is frequently hampered by fibroblasts overgrowing the target cells. For optimal results, the Tumor Cell Isolation Kit, human should be used in combination with the Tumor Dissociation Kit, human (# 130-095-929) and gentleMACS™ Dissociators.

Columns

LS or autoMACS

^®

Columns.

Resources for Tumor Cell Isolation Kit, human

Documents and Protocols

Scientific posters

Tumor cells from human, mouse, and xenografted tumors

Scientific posters

Isolation of primary human tumor cells significantly reduces bias in molecular analysis and improves culture of target cells

Scientific posters

Isolation of primary human tumor cells improves culture of target cells and reduces bias in molecular analysis

Scientific posters

Optimized workflow improves the characterization of tumor-infiltrating T cells

App notes/customer reports

Untouched tumor cell isolation allows for subsequent enrichment of rare cancer stem cells and improves NGS analysis.

Data and images