Name: TexMACS™ Medium
Availability: InStock

Product data and images for TexMACS™ Medium

Figures

Figure 1

**Efficient expansion of human T cells under serum-free conditions**
: Human T cells were isolated using the Pan T cell Isolation Kit, human. Subsequently, cells were stimulated either with T Cell TransAct™, human (research grade), with activation beads (competitor brand), or left untreated and cultured for 14 days either in RPMI (+10% FBS) or serum-free TexMACS™ Medium. Both media were supplemented with 50 U/mL Human IL-2 IS, premium-grade.
Cell proliferation (fold expansion) was analyzed on the MACSQuant
^®
Analyzer 10. Culturing in serum-free TexMACS Medium resulted in over 100-fold T cell expansion comparable to the expansion rates in RPMI + 10% FBS.

Figure 1

Efficient expansion of human T cells under serum-free conditions

: Human T cells were isolated using the Pan T cell Isolation Kit, human. Subsequently, cells were stimulated either with T Cell TransAct™, human (research grade), with activation beads (competitor brand), or left untreated and cultured for 14 days either in RPMI (+10% FBS) or serum-free TexMACS™ Medium. Both media were supplemented with 50 U/mL Human IL-2 IS, premium-grade.

Cell proliferation (fold expansion) was analyzed on the MACSQuant

^®

Analyzer 10. Culturing in serum-free TexMACS Medium resulted in over 100-fold T cell expansion comparable to the expansion rates in RPMI + 10% FBS.

Figure 2

**Reliable expansion of human FoxP3**⁺ **regulatory T cells:**
Human regulatory T cells (Tregs) were isolated directly from whole blood using the MACSxpress
^®
Whole Blood Treg Isolation Kit, human and expanded for 21 days after activation using the Treg Expansion Kit, human. Cells were cultured in TexMACS™ Medium (suppl. with 5% AB serum, 500 U/mL IL-2 IS, Premium-Grade and 100 nmol/L MACS
^®
GMP Rapamycin).
Treg cells expanded up to 40-fold in 21 days while maintaining their FoxP3
⁺
phenotype.

Figure 2

Reliable expansion of human FoxP3⁺ regulatory T cells:

Human regulatory T cells (Tregs) were isolated directly from whole blood using the MACSxpress

^®

Whole Blood Treg Isolation Kit, human and expanded for 21 days after activation using the Treg Expansion Kit, human. Cells were cultured in TexMACS™ Medium (suppl. with 5% AB serum, 500 U/mL IL-2 IS, Premium-Grade and 100 nmol/L MACS

^®

GMP Rapamycin).

Treg cells expanded up to 40-fold in 21 days while maintaining their FoxP3

⁺

phenotype.

Figure 3

**Expansion of mouse CD4**⁺ **T cells with and without addition of bovine serum:**
Mouse CD4
⁺
T cells were isolated on the autoMACS
^®
Pro Separator using the CD4
⁺
T Cell Isolation Kit, mouse, activated via the T Cell Activation/Expansion Kit, mouse, and expanded for 7 days in TexMACS™ Medium with and without FBS. Culture media were supplemented with 50 U/mL Mouse IL-2 IS, premium-grade.
Cell proliferation (fold expansion) was analyzed on the MACSQuant
^®
Analyzer 10. Culturing in serum-free as well as serum-containing TexMACS™ Medium resulted 10–20-fold T cell expansion.

Figure 3

Expansion of mouse CD4⁺ T cells with and without addition of bovine serum:

Mouse CD4

⁺

T cells were isolated on the autoMACS

^®

Pro Separator using the CD4

⁺

T Cell Isolation Kit, mouse, activated via the T Cell Activation/Expansion Kit, mouse, and expanded for 7 days in TexMACS™ Medium with and without FBS. Culture media were supplemented with 50 U/mL Mouse IL-2 IS, premium-grade.

Cell proliferation (fold expansion) was analyzed on the MACSQuant

^®

Analyzer 10. Culturing in serum-free as well as serum-containing TexMACS™ Medium resulted 10–20-fold T cell expansion.

Specifications for TexMACS™ Medium

Overview

TexMACS Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells. This complete medium enables reproducible application in human and mouse cell culture. It is manufactured without animal-derived components.

Detailed product information

Background information

TexMACS Medium has been designed for high-performance T cell growth, high cell viability, and consistency under serum-free conditions. It has been optimized for the serum-free cultivation and expansion of human and mouse T cells and regulatory T cells. TexMACS Medium has a defined formulation enabling reproducible application in human and mouse cell culture. It is produced without animal-derived components, but contains pre-selected human serum albumin, stable glutamine, and phenol red. TexMACS Medium has been developed based on the TexMACS GMP Medium (# 170-076-306).

Resources for TexMACS™ Medium

Documents and Protocols

Brochures/posters

T cell research - The complete product portfolio

Brochures/posters

Get your CAR T cell research moving

App notes/customer reports

Fast and efficient T cell isolation without density gradient centrifugation for CAR T cell engineering

App notes/customer reports

Generation of functional CAR T cells from pan T cells isolated by MACS® technology

App notes/customer reports

Generation of functional CAR T cells from CD4⁺ and CD8⁺ T cells

References for TexMACS™ Medium

Publications

Bacher, P. et al. (2013) Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Tʜ cell repertoire. J Immunol 190(8): 3967-3976
Saito, S. et al. (2014)
Anti-leukemic potency of
piggyBac
-mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia.
Cytotherapy 16: 1257-1269

Data and images