Tandem dyes greatly expand the range of options for multicolor flow cytometry. However, tandem dye–conjugated antibodies often show non-specific binding to cells, in particular monocytes. Using the Tandem Signal Enhancer when staining with tandem-dye–conjugated antibodies, the non-specific binding is strongly reduced. This ready-to-use reagent increases binding specificity of tandem dye–conjugated antibodies to cells in human PBMCs or whole blood. The addition of FcR Blocking Reagent is not required.
