StainExpress™ Immune Cell Composition Cocktail, human
StainExpress™ Immune Cell Composition Cocktail, human
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Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). | |
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 View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |  View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
C: | D: |
 View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |  View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
E: | F: |
 View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |  View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
G: | H: |
 View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |  View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). | |
A: | B: |
View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). | View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
C: | D: |
View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). | View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
E: | F: |
View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). | View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
G: | H: |
View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). | View details StainExpress™ Immune Cell Composition Cocktail, humanFigure 1Whole blood from a healthy donor was stained with the StainExpress Immune Cell Composition Cocktail. Staining was carried out for 10 minutes at room temperature (19−25 °C). Subsequently, red blood cells were lysed by incubation with 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B), and dead cells were excluded by 7-AAD (C). CD3 + cells were identified (D) and discriminated into T cells and NKT cells based on expression of CD56 (E). T cells were further divided into CD4 + and CD8 + T cells (F). Among CD3 – cells, monocytes were defined by CD14 expression and B cells by CD19 expression (G). The remaining CD14 –/CD19 – cells were further devided into SSC high/CD16 – eosinophils, SSC high/CD16 + neutrophils, and SSC low/CD56 +/CD16 + cells (H). |
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