EA.hy926 cells, overnight serum starved and stimulated with 150 nM Calyculin A for 30 minutes, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were stained with REA Control (I) antibodies or with the corresponding Anti-p120 Catenin (pT916) antibodies (right peaks). In case of Vio^® 515 and Vio^® R720, Jurkat cells were fixed, permeabilized, and stained with REA Control (I) antibodies or with the corresponding Anti-Ki-67 antibodies (right peaks). Cells were analyzed by flow cytometry using the MACSQuant^® Analyzer.Cell debris were excluded from the analysis based on scatter signals.

3D-immunofluorescence analysis of a human cerebral organoid and a mouse brain hemisphere fixed with 4% PFA and subjected to whole-mount immunostaining with REA Control Antibody (I), human IgG1, Vio R667 (red). The samples were processed and optically cleared using the MACS^® Clearing Kit and imaged with an UltraMicroscope II light sheet instrument. Human cerebral organoid stained with REA Control Antibody (I), human IgG1, Vio R667 (A) and stained with Sox2 Antibody, anti-human/mouse, Vio R667 (B). Mouse brain hemisphere stained with REA Control Antibody (I), human IgG1, Vio R667 (C) and CD31 Antibody, anti-mouse, Vio R667 (D). Scale bars: 300 µm (A, B), 500 µm (C, D)