NK1.1 Antibody, anti-mouse

Name: NK1.1 Antibody, anti-mouse
Availability: InStock

NK1.1 Antibody, anti-mouseExtended Validation

Clone:

PK136

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2aκ

Applications:

FC, MICS, IF, IHC

Alternative names:

KLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c

Flow cytometry applications forNK1.1 Antibody, anti-mouse

APC
APC-Vio 770
Biotin
PE
PE-Vio 770
PerCP-Vio 700

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-117-528, 130-117-379

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Spleen cells from C57BL/6 mice were fluorescently stained with Anti-NK1.1 antibodies as well as with Anti-NKp46 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

MICS (MACSima Imaging Cyclic Staining) applications forNK1.1 Antibody, anti-mouse

APC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

mouse

Products used:

130-117-528, 130-117-379

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for NK1.1 Antibody, anti-mouse

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with PK136
REA1162	++
694370	+
Cells were incubated with an excess of purified unconjugated NK1.1 (PK136) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (PK136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (PK136). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for NK1.1 Antibody, anti-mouse

Overview

The PK136 monoclonal antibody reacts with NK1.1, also known as CD161. NK1.1 is expressed by natural killer cells and a subset of T cells in the mouse strains C57BL/6, FVB/N, and NZB, but not in the mouse strains AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129.

Alternative names

KLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c

Detailed product information

Technical specifications

Clone	PK136
Clonality	monoclonal
Isotype	mouse IgG2aκ
Isotype control	Isotype Control Antibody, mouse IgG2a
Host	mouse
Type of antibody	Primary antibodies
Species	mouse
Antigen	NK1.1
Alternative names of antigen	KLRB1C, CD161, Ly-59, NK-RP1, NK-1, Nk-1.2, Nkrp1c, Ly-55c
Molecular mass of antigen [kDa]	25
Distribution of antigen	T cells, NK cells
Entrez Gene ID	17059
RRID	AB_2727975, AB_2727927, AB_2727573, AB_2727596, AB_2733163, AB_2733164, AB_2728038, AB_2728015, AB_2660600, AB_2660601, AB_2727578

Resources for NK1.1 Antibody, anti-mouse

Certificates

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Applications

Extended validation