MACS
^®
GMP Recombinant Human TNF-α

Name: MACS® GMP Recombinant Human TNF-α
Availability: InStock

MACS
^®
GMP Recombinant Human TNF-α

Tumor necrosis factor alpha (TNF-α) has a broad spectrum of biological activities. In addition to its central role in inflammation TNF-α is involved in regulating apoptotic cell death, cellular proliferation, and differentiation.

MACS GMP Recombinant Human TNF-α is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human TNF-α

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TNF-α. The peak corresponds to the calculated molecular mass of 17353 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TNF-α. The peak corresponds to the calculated molecular mass of 17353 Da.

Figure 2

**MACS GMP Recombinant Human TNF-α biological activity.**
Activity of Human TNF-α, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human TNF-α biological activity.

Activity of Human TNF-α, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

**MACS GMP Recombinant Human TNF-α batch-to-batch variability.**
Activity of three different production batches of Human MACS GMP TNF-α.

Figure 3

MACS GMP Recombinant Human TNF-α batch-to-batch variability.

Activity of three different production batches of Human MACS GMP TNF-α.

Specifications for MACS
^®
GMP Recombinant Human TNF-α

Overview

Tumor necrosis factor alpha (TNF-α) has a broad spectrum of biological activities. In addition to its central role in inflammation TNF-α is involved in regulating apoptotic cell death, cellular proliferation, and differentiation.

MACS GMP Recombinant Human TNF-α is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

TNF-α is mainly produced by activated monocytes and macrophages, but also by a broad variety of other cell types including lymphoid cells, mast cells, endothelial cells, fibroblasts, and astrocytes. Beside its primary role in immune regulation, TNF-α promotes angiogenesis, bone resorption, and thrombotic processes.

Applications

MACS GMP Recombinant Human TNF-α can be used for a variety of applications, including the

ex vivo

generation of human dendritic cells from enriched CD14

⁺

monocyte populations.

^2–4

Biological activity

The specific activity is determined by cytotoxicity assay using L929 cells provided by the German Collection of Microorganisms and Cell Cultures (DSMZ) in the presence of 1 μg/mL actinomycin D according to Baarsch
et al
.
¹
The cytotoxicity assay was calibrated with the international standard for human TNF-α (NIBSC code 88/786) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 2×
10
⁷
IU/mg
Typical specific activity: ≥ ≥ 3.9×
10
⁷
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
^®
GMP Recombinant Human TNF-α

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human TNF-α

Publications

Baarsch, M. J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140: 15-22
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit, H. et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

Data and images