MACS® GMP Recombinant Human SCF

Stem cell factor (SCF), also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF) is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration.
MACS GMP Recombinant Human SCF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective

Data and images for
MACS
®
GMP Recombinant Human SCF

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human SCF. The peak corresponds to the calculated molecular mass of 18585 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human SCF. The peak corresponds to the calculated molecular mass of 18585 Da.

Figure 2

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MACS GMP Recombinant Human SCF biological activity.
Activity of Human SCF, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human SCF biological activity.
Activity of Human SCF, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Specifications for
MACS
®
GMP Recombinant Human SCF

Overview

Stem cell factor (SCF), also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF) is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration.
MACS GMP Recombinant Human SCF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. SCF is constitutively produced by endothelial cells and by stromal fibroblasts.

Applications

MACS GMP Recombinant Human SCF can be used for a variety of applications, including the expansion
2,3
and the viral transduction
4,5
of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood, or the
in vitro
differentiation of megakaryocytes
6
.
Technical specifications
  • Identity/ Molecular mass: 18.585 Da, determined by mass spectrometry. This corresponds to the mature form of human SCF including an N-terminal methionine (165 amino acids).
  • Purity: ≥ 95 % as determined by chip electrophoresis.
  • Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
  • Residual host cell DNA content: < 5 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
  • Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.
We have adapted the product quality certificate of our MACS
®
GMP Recombinant Human SCF. Learn more
here

Biological activity

  • The specific activity is determined by proliferation assay according to Kitamura
    et al.1
    using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human SCF (NIBSC code 91/682) provided by the National Institute for Biological Standards and Control.
  • Minimal specific activity: ≥ ≥ 5×
    10
    5
    U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for
ex vivo
cell processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

References for
MACS
®
GMP Recombinant Human SCF

Publications

  1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  2. Broudy, V. C. (1997) Stem cell factor and hematopoiesis. Blood 90(4): 1345-1364
  3. Petzer, A. L. et al. (1996)
    Differential cytokine effects on primitive (CD34
    +
    CD38
    -
    ) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.
    J. Exp. Med. 183(6): 2551-2558
  4. Kiem, H. P. et al. (1998) Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood 92(6): 1878-1886
  5. Uchida, N. et al. (2011)
    Optimal conditions for lentiviral transduction of engrafting human CD34
    +
    cells.
    Gene Ther. 18(11): 1078-1086
  6. Bruno, S. et al. (2003)
    In vitro
    and
    in vivo
    megakaryocyte differentiation of fresh and
    ex-vivo
    expanded cord blood cells: rapid and transient megakaryocyte reconstitution.
    Haematologica 88(4): 379-387

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®
GMP Recombinant Human SCF

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