MACS
^®
GMP Recombinant Human M-CSF

Name: MACS® GMP Recombinant Human M-CSF
Availability: InStock

MACS
^®
GMP Recombinant Human M-CSF

M-CSF stands for macrophage colony-stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

MACS GMP Recombinant Human M-CSF is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human M-CSF

Figures

Figure 1

The biological activity of Human M-CSF was determined by a proliferation assay using NFS-60 cells.

Figure 1

The biological activity of Human M-CSF was determined by a proliferation assay using NFS-60 cells.

Figure 2

Mass spectrometry analysis (ESI-MS) of MACS Recombinant Human M-CSF. The peak corresponds to the calculated molecular mass of 36792 Da.

Figure 2

Mass spectrometry analysis (ESI-MS) of MACS Recombinant Human M-CSF. The peak corresponds to the calculated molecular mass of 36792 Da.

Specifications for MACS
^®
GMP Recombinant Human M-CSF

Overview

M-CSF stands for macrophage colony-stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

MACS GMP Recombinant Human M-CSF is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Macrophage colony-stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages, and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

^2–5

Applications

Human M-CSF can be used for a variety of applications, including:

Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
Differentiation of macrophages from peripheral blood monocytes.
Differentiation of osteoclasts from CD14⁺ monocytes.

Technical specifications

Identity/Molecular mass: 36793 Da, determined by mass spectrometry. This corresponds to the mature form of human M-CSF (158 amino acids).
Purity: ≥ 90 % as determined by chip electrophoresis.
Product-related proteins : ≤15% as determined by chip electrophoresis.
Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of MACS

^®

GMP Recombinant Human M-CSF was adapted. Learn more

here.

Biological activity

The specific activity is determined by proliferation assay using mouse M-NFS-60 cells according to Mire-Sluis, A. R.
et al.¹
The proliferation assay was calibrated with the international standard for human M-CSF (NIBSC code 89/512) provided by the WHO/ National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 4×
10
⁷
IU/mg
Typical specific activity: ≥ ≥ 6×
10
⁷
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
^®
GMP Recombinant Human M-CSF

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human M-CSF

Publications

Mire-Sluis, A. R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
Guery, L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
Stanley, E. R. et al. (1997) Biology and action of colony-stimulating factor-1. Mol. Reprod. Dev. 46(1): 4-10
Fixe, P. et al. (1997) Macrophage colony-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships. Eur. Cytokine Netw. 8(2): 125-136

Data and images

Data and images for MACS
^®
GMP Recombinant Human M-CSF

Figures

Figure 1

The biological activity of Human M-CSF was determined by a proliferation assay using NFS-60 cells.

Figure 2

Mass spectrometry analysis (ESI-MS) of MACS Recombinant Human M-CSF. The peak corresponds to the calculated molecular mass of 36792 Da.

Specifications

Specifications for MACS
^®
GMP Recombinant Human M-CSF

Overview

M-CSF stands for macrophage colony-stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

MACS GMP Recombinant Human M-CSF is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Macrophage colony-stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages, and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

^2–5

Applications

Human M-CSF can be used for a variety of applications, including:

Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
Differentiation of macrophages from peripheral blood monocytes.
Differentiation of osteoclasts from CD14⁺ monocytes.

Technical specifications

Identity/Molecular mass: 36793 Da, determined by mass spectrometry. This corresponds to the mature form of human M-CSF (158 amino acids).
Purity: ≥ 90 % as determined by chip electrophoresis.
Product-related proteins : ≤15% as determined by chip electrophoresis.
Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of MACS

^®

GMP Recombinant Human M-CSF was adapted. Learn more

here.

Biological activity

The specific activity is determined by proliferation assay using mouse M-NFS-60 cells according to Mire-Sluis, A. R.
et al.¹
The proliferation assay was calibrated with the international standard for human M-CSF (NIBSC code 89/512) provided by the WHO/ National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 4×
10
⁷
IU/mg
Typical specific activity: ≥ ≥ 6×
10
⁷
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources

Resources for MACS
^®
GMP Recombinant Human M-CSF

Documents and Protocols

Brochures/posters

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References

References for MACS
^®
GMP Recombinant Human M-CSF

Publications

Mire-Sluis, A. R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
Guery, L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
Stanley, E. R. et al. (1997) Biology and action of colony-stimulating factor-1. Mol. Reprod. Dev. 46(1): 4-10
Fixe, P. et al. (1997) Macrophage colony-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships. Eur. Cytokine Netw. 8(2): 125-136

Related products for

MACS
^®
GMP Recombinant Human M-CSF

1 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?

MACS® GMP Recombinant Human M-CSF

MACS® GMP Recombinant Human M-CSF

Product data and images for MACS® GMP Recombinant Human M-CSF

Figures

Figure 1