MACS
^®
GMP Recombinant Human IL-6

Name: MACS® GMP Recombinant Human IL-6
Availability: InStock

MACS
^®
GMP Recombinant Human IL-6

Interleukin 6 (IL-6) is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.

MACS GMP Recombinant Human IL-6 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human IL-6

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-6. The peak corresponds to the calculated molecular mass of 20812 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-6. The peak corresponds to the calculated molecular mass of 20812 Da.

Figure 2

**MACS GMP Recombinant Human IL-6 biological activity.**
Activity of Human IL-6, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Figure 2

MACS GMP Recombinant Human IL-6 biological activity.

Activity of Human IL-6, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Specifications for MACS
^®
GMP Recombinant Human IL-6

Overview

Interleukin 6 (IL-6) is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.

MACS GMP Recombinant Human IL-6 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-6 is mainly produced by monocytes and macrophages, but as well by fibroblasts or endothelial cells. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 induces the terminal maturation of activated B cells into plasma cells, and in combination with other factors like IL-2 and IFN-γ it also affects cytotoxic T lymphocytes.

Applications

MACS GMP Recombinant Human IL-6 can be used for a variety of applications, including the

ex vivo

generation of human dendritic cells from enriched CD14

⁺

monocytes.

^2–4

Biological activity

The specific activity is determined by proliferation assay according to Gaines-Das and Poole
¹
using B9 hybridoma cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human IL-6 (NIBSC code 89/548) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 8×
10
⁷
IU/mg
Typical specific activity: ≥ ≥ 1.2×
10
⁸
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
^®
GMP Recombinant Human IL-6

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

Scientific posters

Blood DC preparations generated using automated CliniMACS Prodigy^®CD1c/CD304 enrichment and activation system efficiently activate CD8⁺ antigen-specific T cells

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human IL-6

Publications

Gaines-Das, R. E. and Poole, S. (1993) The international standard for interleukin-6. Evaluation in an international collaborative study. J. Immunol. Methods 160: 147-153
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit, H. et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

Data and images

Data and images for MACS
^®
GMP Recombinant Human IL-6

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-6. The peak corresponds to the calculated molecular mass of 20812 Da.

Figure 2

MACS GMP Recombinant Human IL-6 biological activity.

Activity of Human IL-6, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Specifications

Specifications for MACS
^®
GMP Recombinant Human IL-6

Overview

Interleukin 6 (IL-6) is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.

MACS GMP Recombinant Human IL-6 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-6 is mainly produced by monocytes and macrophages, but as well by fibroblasts or endothelial cells. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 induces the terminal maturation of activated B cells into plasma cells, and in combination with other factors like IL-2 and IFN-γ it also affects cytotoxic T lymphocytes.

Applications

MACS GMP Recombinant Human IL-6 can be used for a variety of applications, including the

ex vivo

generation of human dendritic cells from enriched CD14

⁺

monocytes.

^2–4

Biological activity

The specific activity is determined by proliferation assay according to Gaines-Das and Poole
¹
using B9 hybridoma cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human IL-6 (NIBSC code 89/548) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 8×
10
⁷
IU/mg
Typical specific activity: ≥ ≥ 1.2×
10
⁸
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources

Resources for MACS
^®
GMP Recombinant Human IL-6

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

Scientific posters

Blood DC preparations generated using automated CliniMACS Prodigy^®CD1c/CD304 enrichment and activation system efficiently activate CD8⁺ antigen-specific T cells

App notes/customer reports

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References

References for MACS
^®
GMP Recombinant Human IL-6

Publications

Gaines-Das, R. E. and Poole, S. (1993) The international standard for interleukin-6. Evaluation in an international collaborative study. J. Immunol. Methods 160: 147-153
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit, H. et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

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^®
GMP Recombinant Human IL-6

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MACS® GMP Recombinant Human IL-6

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Product data and images for MACS® GMP Recombinant Human IL-6

Figures

Figure 1