MACS
^®
GMP Recombinant Human IL-4

Name: MACS® GMP Recombinant Human IL-4
Availability: InStock

MACS
^®
GMP Recombinant Human IL-4

Interleukin 4 (IL-4) is an anti-inflammatory cytokine and is a key regulator in humoral and adaptive immunity. It has complex biological roles and promotes growth and differentiation of B cells and T cells, and cells of the monocytic lineage.

MACS GMP Recombinant Human IL-4 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human IL-4

Figures

Figure 1

The biological activity of MACS GMP Recombinant Human IL-4 was determined by proliferation assay using TF-1 cells.

Figure 1

The biological activity of MACS GMP Recombinant Human IL-4 was determined by proliferation assay using TF-1 cells.

Figure 2

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-4. The peak corresponds to the calculated molecular mass of 15088 Da.

Figure 2

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-4. The peak corresponds to the calculated molecular mass of 15088 Da.

Figure 3

MACS GMP Recombinant Human IL-4 batch-to-batch variability. Activity of three different production batches of Human MACS GMP IL-4.

Figure 3

MACS GMP Recombinant Human IL-4 batch-to-batch variability. Activity of three different production batches of Human MACS GMP IL-4.

Specifications for MACS
^®
GMP Recombinant Human IL-4

Overview

Interleukin 4 (IL-4) is an anti-inflammatory cytokine and is a key regulator in humoral and adaptive immunity. It has complex biological roles and promotes growth and differentiation of B cells and T cells, and cells of the monocytic lineage.

MACS GMP Recombinant Human IL-4 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-4 is predominantly secreted by activated CD4

⁺

memory and effector T

_H

2 cells, basophils, and mast cells. It promotes the proliferation and differentiation of B cells, as well as immunoglobulin isotype switching, and MHC class II antigen and low-affinity IgE receptor expression. Furthermore, IL-4 induces the differentiation of naive CD4

⁺

T cells into helper T

_H

2 cells, while suppressing T

_H

1 development, and promotes chemotaxis of mast cells and basophils.

Applications

MACS GMP Recombinant Human IL-4 can be used for a variety of applications, including the

ex vivo

generation of human dendritic cells from enriched CD14

⁺

monocyte populations.

^2–⁴

Biological activity

The specific activity is determined by proliferation assay according to Kitamura
et al.¹
using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human IL-4 (NIBSC code 88/656) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 2×
10
⁶
IU/mg
Typical specific activity: ≥ ≥ 4.7×
10
⁶
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
^®
GMP Recombinant Human IL-4

Documents and Protocols

Scientific posters

Monocyte-derived dendritic cells (Mo-DCs) generated with CliniMACS Prodigy® are functional and fulfill requirements for cancer vaccines

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human IL-4

Publications

Kitamura, T. et al. (1991) IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoetic cell line, TF-1. Int. Immunol. 3: 571-577
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit, H. et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

Data and images