MACS
^®
GMP Recombinant Human IL-21

Name: MACS® GMP Recombinant Human IL-21
Availability: InStock

MACS
^®
GMP Recombinant Human IL-21

Interleukin 21 (IL-21) has pleiotropic effects on both cellular and humoral immune responses, such as stimulation of lymphocyte proliferation, promotion of CD8

⁺

T cell and NK cell cytotoxicity, and differentiation of B cells into plasma cells.

MACS GMP Recombinant Human IL-21 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for manufacture of this product. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human IL-21

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-21. The peak corresponds to the calculated molecular mass of 15460 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-21. The peak corresponds to the calculated molecular mass of 15460 Da.

Figure 2

The biological activity of MACS GMP Recombinant Human IL-21 was determined by a proliferation assay using mouse B9 hybridoma cells.

Figure 2

The biological activity of MACS GMP Recombinant Human IL-21 was determined by a proliferation assay using mouse B9 hybridoma cells.

Figure 3

**MACS GMP Recombinant Human IL-21 biological activity.**
Activity of Human MACS GMP IL-21 (blue bar), was compared to another commercially available product (black bar).

Figure 3

MACS GMP Recombinant Human IL-21 biological activity.

Activity of Human MACS GMP IL-21 (blue bar), was compared to another commercially available product (black bar).

Specifications for MACS
^®
GMP Recombinant Human IL-21

Overview

Interleukin 21 (IL-21) has pleiotropic effects on both cellular and humoral immune responses, such as stimulation of lymphocyte proliferation, promotion of CD8

⁺

T cell and NK cell cytotoxicity, and differentiation of B cells into plasma cells.

MACS GMP Recombinant Human IL-21 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for manufacture of this product. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-21 is a four α-helix bundle cytokine and closely related to IL-2, IL-7, and IL-15. IL-21 expression is restricted to activated CD4

⁺

T helper cells and NKT cells. The functional receptor for IL-21, composed of the IL-21 receptor- and the common γ-chain, is expressed on various hematopoietic cells including T, B, NK and dendritic cells.

Applications

MACS GMP Recombinant Human IL-21 can be used for a variety of applications, including the

ex vivo

generation of antigen-specific cytotoxic T cells

^1,2

or the

ex vivo

stimulation of NK cells

³

.

Technical specifications

Identity/Molecular mass: 15460 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-21 (133 amino acids).
Purity: ≥ 95 % as determined by chip electrophoresis.
Endotoxin content: < 100 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of MACS

^®

GMP Recombinant Human IL-21 was adapted. Learn more

here.

Biological activity

The specific activity is determined by proliferation assay using mouse B9 hybridoma cells provided by the German Resource Centre for Biological Material (DSMZ).
Minimal specific activity: ≥ 1×
10
⁴
U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
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GMP Recombinant Human IL-21

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human IL-21

Publications

Li, Y. and Yee, C. (2008)
IL-21 mediated Foxp3 suppression leads to enhanced generation of antigen-specific CD8
⁺
cytotoxic T lymphocytes.
Blood 111: 229-235
Hinrichs, C. S. et al. (2008)
IL-2 and IL-21 confer opposing differentiation programs to CD8
⁺
T cells for adoptive immunotherapy.
Blood 111: 5326-5333
de Rham, C. et al. (2007) The proinflammatory cytokines IL-2, IL-15 and IL-21 modulate the repertoire of mature human natural killer cell receptors. Arthritis Res. Ther. 9: R125

Data and images