MACS
^®
GMP Recombinant Human IL-2

Name: MACS® GMP Recombinant Human IL-2
Availability: InStock

MACS
^®
GMP Recombinant Human IL-2

Interleukin 2 (IL-2), a potent lymphoid cell growth factor, plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes, for instance, proliferation and differentiation of T cells, NK cells, and B cells.

MACS GMP Recombinant Human IL-2 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human IL-2

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-2. The peak corresponds to the calculated molecular mass of 15400 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-2. The peak corresponds to the calculated molecular mass of 15400 Da.

Figure 2

**MACS GMP Recombinant Human IL-2 biological activity.**
Activity of Human IL-2, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human IL-2 biological activity.

Activity of Human IL-2, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

**MACS GMP Recombinant Human IL-2 batch-to-batch variability.**
Activity of three different production batches of Human MACS GMP IL-2.

Figure 3

MACS GMP Recombinant Human IL-2 batch-to-batch variability.

Activity of three different production batches of Human MACS GMP IL-2.

Specifications for MACS
^®
GMP Recombinant Human IL-2

Overview

Interleukin 2 (IL-2), a potent lymphoid cell growth factor, plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes, for instance, proliferation and differentiation of T cells, NK cells, and B cells.

MACS GMP Recombinant Human IL-2 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-2 is a typical four α-helix bundle cytokine and is produced by activated T cells, especially the CD4

⁺

T helper cell population. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β-chain, and common γ-chain.

Applications

MACS GMP Recombinant Human IL-2 can be used for a variety of applications, including the

ex vivo

activation and expansion of T cells, e.g., antigen-specific cytotoxic T lymphocytes

²^,³

or regulatory T cells

⁴

or the

ex vivo

stimulation of NK cells

⁵^,⁶

.

Biological activity

The specific activity is determined by proliferation assay according to Gearing and Bird
¹
using CTLL-2 cells. The proliferation assay was calibrated with the 2nd international standard for human IL-2 (NIBSC code 86/500) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 1×
10
⁶
IU/mg
Typical specific activity: ≥ ≥ 5×
10
⁶
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
^®
GMP Recombinant Human IL-2

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

A case study: Cytokine cost calculation for a T cell transduction run

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human IL-2

Publications

Gearing, A. J. H. and Bird, C. B. (1987) Lymphokines and interferons, a practical approach. Oxford: IRL Press : 295
Zhang, H. et al. (2007) 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy. J Immunol 179: 4910-4918
Hinrichs, C. S. et al. (2008)
IL-2 and IL-21 confer opposing differentiation programs to CD8
⁺
T cells for adoptive immunotherapy.
Blood 111: 5326-5333
Peters, J. H. et al. (2008)
Ex vivo
generation of human alloantigen-specific regulatory T cells from CD4
⁺
CD25
^high
T cells for immunotherapy.
PLoS One 3(5): e2233
Berg, M. et al. (2009)
Clinical-grade
ex vivo
-expanded human natural killer cells up-regulate activating receptors and death receptor ligands and have enhanced cytolytic activity against tumor cells.
Cytotherapy 11: 341-355
McKenna, D. H. Jr. et al. (2007) Good manufacturing practices production of natural killer cells for immunotherapy: a six-year single-institution experience. Transfusion 47(3): 520-528

Data and images