MACS
^®
GMP Recombinant Human IL-1β

Name: MACS® GMP Recombinant Human IL-1β
Availability: InStock

MACS
^®
GMP Recombinant Human IL-1β

Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that mediates inflammatory responses and displays a wide variety of biological activities on many different cell types, including T cells, B cells, and monocytes.

MACS GMP Recombinant Human IL-1β is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Product data and images for MACS
^®
GMP Recombinant Human IL-1β

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-1β. The peak corresponds to the calculated molecular mass of 17377 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-1β. The peak corresponds to the calculated molecular mass of 17377 Da.

Figure 2

**MACS GMP Recombinant Human IL-1β biological activity.**
Activity of Human IL-1β, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human IL-1β biological activity.

Activity of Human IL-1β, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Specifications for MACS
^®
GMP Recombinant Human IL-1β

Overview

Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that mediates inflammatory responses and displays a wide variety of biological activities on many different cell types, including T cells, B cells, and monocytes.

MACS GMP Recombinant Human IL-1β is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-1β is mainly secreted by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. IL-1β acts also on nonimmune system cells such as fibroblasts, vascular endothelial cells, and hepatocytes.

Applications

MACS GMP Recombinant Human IL-1β can be used for a variety of applications, including the

ex vivo

generation of human dendritic cells from enriched CD14

⁺

monocyte populations.

^2–4

Technical specifications

Identity/ Molecular mass: 17377 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-1β (153 amino acids).
Purity: ≥ 97 % as determined by chip electrophoresis.
Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
Residual host cell DNA content: < 5 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
Residual host cell protein content: < 0.5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of MACS

^®

GMP Recombinant Human IL-1β was adapted. Learn more

here.

Biological activity

The specific activity is determined by proliferation assay according to Poole and Gaines-Das
¹
using D10.G4.1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the first international reference standard for human IL-1β (NIBSC code 86/680) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 2×
10
⁷
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS
^®
GMP Recombinant Human IL-1β

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

The reliability of stated cytokine activities

App notes/customer reports

Your guide to side-by-side cytokine comparison

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

App notes/customer reports

Recombinant cytokines and their biological activity

App notes/customer reports

Bioassays and calibration reagents used for activity calibration of MACS Cytokines

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References for MACS
^®
GMP Recombinant Human IL-1β

Publications

Poole, S. and Gaines-Das, R. E. (1991) The international standards for interleukin-1 alpha and interleukin-1 beta. Evaluation in an international collaborative study. J. Immunol. Methods 142: 1-13
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit, H. et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

Data and images

Data and images for MACS
^®
GMP Recombinant Human IL-1β

Figures

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-1β. The peak corresponds to the calculated molecular mass of 17377 Da.

Figure 2

MACS GMP Recombinant Human IL-1β biological activity.

Activity of Human IL-1β, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Specifications

Specifications for MACS
^®
GMP Recombinant Human IL-1β

Overview

Interleukin-1β (IL-1β) is a pro-inflammatory cytokine that mediates inflammatory responses and displays a wide variety of biological activities on many different cell types, including T cells, B cells, and monocytes.

MACS GMP Recombinant Human IL-1β is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-1β is mainly secreted by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. IL-1β acts also on nonimmune system cells such as fibroblasts, vascular endothelial cells, and hepatocytes.

Applications

MACS GMP Recombinant Human IL-1β can be used for a variety of applications, including the

ex vivo

generation of human dendritic cells from enriched CD14

⁺

monocyte populations.

^2–4

Technical specifications

Identity/ Molecular mass: 17377 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-1β (153 amino acids).
Purity: ≥ 97 % as determined by chip electrophoresis.
Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
Residual host cell DNA content: < 5 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
Residual host cell protein content: < 0.5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of MACS

^®

GMP Recombinant Human IL-1β was adapted. Learn more

here.

Biological activity

The specific activity is determined by proliferation assay according to Poole and Gaines-Das
¹
using D10.G4.1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the first international reference standard for human IL-1β (NIBSC code 86/680) provided by the National Institute for Biological Standards and Control.
Minimal specific activity: ≥ ≥ 2×
10
⁷
IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for

ex vivo

cell processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources

Resources for MACS
^®
GMP Recombinant Human IL-1β

Documents and Protocols

Brochures/posters

Certificates

Please follow this

link

to search for Product Quality Certificates (PQC) by lot number.

References

References for MACS
^®
GMP Recombinant Human IL-1β

Publications

Poole, S. and Gaines-Das, R. E. (1991) The international standards for interleukin-1 alpha and interleukin-1 beta. Evaluation in an international collaborative study. J. Immunol. Methods 142: 1-13
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit, H. et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

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^®
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MACS® GMP Recombinant Human IL-1β

MACS® GMP Recombinant Human IL-1β

Product data and images for MACS® GMP Recombinant Human IL-1β

Figures

Figure 1