MACS® GMP Recombinant Human Flt3-Ligand

Fms-related tyrosine kinase 3 ligand (Flt3-Ligand) is a growth factor that exerts pleiotropic and potent effects on the development of hematopoietic stem cells (HSCs) and the immune system. In synergy with other growth factors Flt3-Ligand promotes proliferation and development of HSCs, myeloid and lymphoid progenitor cells, dendritic cells, and natural killer cells.
MACS GMP Recombinant Human Flt3-Ligand is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The

Data and images for
MACS
®
GMP Recombinant Human Flt3-Ligand

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human Flt3-Ligand. The peak corresponds to the calculated molecular mass of 17934 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human Flt3-Ligand. The peak corresponds to the calculated molecular mass of 17934 Da.

Figure 2

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MACS GMP Recombinant Human Flt3-Ligand biological activity.
Activity of Human Flt3-Ligand, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Figure 2

MACS GMP Recombinant Human Flt3-Ligand biological activity.
Activity of Human Flt3-Ligand, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Figure 3

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MACS GMP Recombinant Human Flt3-Ligand batch-to-batch variability.
Activity of three different production batches of Human MACS GMP Flt3-Ligand.

Figure 3

MACS GMP Recombinant Human Flt3-Ligand batch-to-batch variability.
Activity of three different production batches of Human MACS GMP Flt3-Ligand.

Specifications for
MACS
®
GMP Recombinant Human Flt3-Ligand

Overview

Fms-related tyrosine kinase 3 ligand (Flt3-Ligand) is a growth factor that exerts pleiotropic and potent effects on the development of hematopoietic stem cells (HSCs) and the immune system. In synergy with other growth factors Flt3-Ligand promotes proliferation and development of HSCs, myeloid and lymphoid progenitor cells, dendritic cells, and natural killer cells.
MACS GMP Recombinant Human Flt3-Ligand is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Flt3-Ligand belongs to a small family of α-helical cytokines and is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin.

Applications

MACS GMP Recombinant Human Flt3-Ligand can be used for a variety of applications, including the expansion
2,3
and the viral transduction
4,5
of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood.

Biological activity

  • The specific activity is determined by proliferation assay according to Meyer and Drexler
    1
    using OCI-AML5 cells. The proliferation assay was calibrated with the reference standard for human Flt3-Ligand (NIBSC 96/532) provided by the National Institute for Biological Standards and Control.
  • Minimal specific activity: ≥ 4×
    10
    5
    IU/mg
  • Typical specific activity: ≥ 7.7×
    10
    5
    IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for
ex vivo
cell processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

References for
MACS
®
GMP Recombinant Human Flt3-Ligand

Publications

  1. Meyer, C. and Drexler, H. G. (1999) FLT3 ligand inhibits apoptosis and promotes survival of myeloid leukemia cell lines. Leuk. Lymphoma 32: 577-581
  2. Gilliland, D. G. and Griffin, J. D. (2002) The roles of FLT3 in hematopoiesis and leukemia. Blood 100(5): 1532-1542
  3. Petzer, A. L. et al. (1996)
    Differential cytokine effects on primitive (CD34
    +
    CD38
    -
    ) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.
    J. Exp. Med. 183(6): 2551-2558
  4. Kiem, H. P. et al. (1998) Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood 92(6): 1878-1886
  5. Uchida, N. et al. (2011)
    Optimal conditions for lentiviral transduction of engrafting human CD34
    +
    cells.
    Gene Ther. 18(11): 1078-1086

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®
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