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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Our MACSPlex Mix products allow you to combine individual analytes within one application category according to your needs. This option is available for the MACSPlex Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human, and the MACSPlex Cytokine Kit, mouse.
To put together your MACSPlex Mix, you need the following components:
Because each single reagent has a specific fluorescence property, the combination of analytes is only possible within one defined application category. Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human and the MACSPlex Cytokine Kit, mouse cannot be mixed within a single experiment.
Overview of combinable reagents and necessary software versions to run Express Modes
Application category | Available target analytes | Standard | Basic Kit |
Human Cytokine 12 | IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α | MACSPlex Cytokine 12 Standard, human (130-106-197) | MACSPlex Cytokine Basic Kit, human and mouse (130-109-701) |
Mouse Cytokine 10 | IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α | MACSPlex Cytokine 10 Standard, mouse (130-106-198) | |
Human Cytotoxic T/NK Cell | IL-2, IL-4, IL-6, IL-10, IL17A, IL-21, GM-CSF, Granzyme B, IFN-γ, MCP-1, TNF-α, Perforin | MACSPlex Mix Cytotoxic Standard (130-125-766) | MACSPlex Mix Cytotoxic Basic Kit (130-125-767) |
For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.
ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.
For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays.
30-day trial versions are available for both Flowlogic Software and the Beadlogic Add-on.
Additionally, third-party software can be used for analysis.
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