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Miltenyi Biotec distribution:
PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, stimulated and unstimulated cells were analyzed by using the IL-17 Secretion Assay – Detection Kit (PE) or (APC). Secretion of IL-17 by CD154+
cells is demonstrated. Data were gated on viable CD4+
IL-17 Secretion Assay - Detection Kit (PE)
IL-17 Secretion Assay - Detection Kit (APC)