CD4 (Domain 2) antibodies, rat

CD4 (Domain 2) antibodies, rat

Clone: REA489 | Dilution: 1:10
Clone REA489 recognizes domain 2 of the rat CD4 antigen, a single-pass type I membrane protein, also known as T cell surface antigen T4/Leu-3 or W3/25 antigen. CD4 is a member of the immunoglobulin superfamily and is expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It has four immunoglobulin domains (D1 to D4). D1 and D3 resemble immunoglobulin variable (IgV) domains, and D2 and D4 resemble immunoglobulin constant (IgC) domains. CD4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell (APC). CD4 amplifies the signal generated by the TCR by recruiting the tyrosine kinase Lck, which is essential for activating many molecular components of the signaling cascade of an activated T cell. CD4 also interacts directly with MHC class II molecules on the surface of APCs.
Additional information: Clone REA489 displays negligible binding to Fc receptors.

Alternative names

W3/25, p55, Leu-3

Technical specifications

  • Antigen: CD4
  • Clone: REA489
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: W3/25, p55, Leu-3
  • Entrez Gene ID: 24932
  • Molecular mass of antigen [kDa]: 48
  • Distribution of antigen: dendritic cells, macrophages, monocytes, T cells, thymocytes, T helper cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected references

    1. Clark, S. J. et al. (1987) Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains. Proc. Natl. Acad. Sci. U.S.A. 84(6): 1649-1653
    2. Almolda, B. et al. (2009) CD4 microglial expression correlates with spontaneous clinical improvement in the acute Lewis rat EAE model. J. Neuroimmunol. 209(1-2): 65-80
    3. Jin, H. et al. (2013)
      CD4
      +
      CD25
      +
      Foxp3
      +
      T cells contribute to the antiasthmatic effects of
      Astragalus membranaceus
      extract in a rat model of asthma.
      Int. Immunopharmacol. 15(1): 42-49
Product options: 14
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9 µg in 300 µL (1)
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9 µg in 300 µL (1)
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30 µg in 1 mL (1)
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9 µg in 300 µL (1)
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30 µg in 1 mL (1)
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9 µg in 300 µL (1)
EUR 22,00 
30 µg in 1 mL (1)
EUR 40,00 

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