CD178 antibodies – functional grade, mouse

CD178 antibodies – functional grade, mouse

Clone: MFL3
The monoclonal antibody MFL3 recognizes the type II transmembrane glycoprotein mouse CD178, also known as Fas Ligand (FasL) or CD95 Ligand (CD95L), which is a 40 kDa member of the TNF cytokine family expressed on activated lymphocytes such as T cells, NK cells, or monocytes as well as in immune-privilaged sites, e.g cornea or testis. CD178 can trigger apoptosis through trimerization of the Fas receptor CD95. Thereby, CD178 and CD95 play a key role in the regulation of the immune system. Cleavage of membrane bound CD178 from the cell surface via matrix metalloproteinases (such as MMP-7) generates soluble CD178 molecules.

Applications

This pure – functional grade antibody is suited for cell culture functional assays, for example, blocking of receptor-ligand binding.

Alternative names

FasL, APT1LG1, CD95L, TNFSF6, gld

Technical specifications

  • Antigen: CD178
  • Clone: MFL3
  • Isotype: hamster IgG
  • Alternative names of antigen: FasL, APT1LG1, CD95L, TNFSF6, gld
  • Distribution of antigen: T cells, monocytes, NK cells
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Janssen, O. et al. (2003) CD95 ligand – death factor and costimulatory molecule? Cell Death Differ. 10(11): 1215-1225
    2. Oyaizu, N. et al. (1997)
      Monocytes express Fas ligand upon CD4 cross-linking and induce CD4
      +
      T cells apoptosis: a possible mechanism of bystander cell death in HIV infection.
      J. Immunol. 158: 2456-2463
    3. Kayagaki, N. et al. (1997) Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. U.S.A. 94: 3914-3919
    4. Takahashi, T. et al. (1994) Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand. Cell 76(6): 969-976
Product options: 1
500 µg in 0.5 mL
EUR 262,00