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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Splenocytes from C57BL/6 mice were either left unstimulated (left image) or stimulated with 0.5 µg/mL ionomycin, 0.5 µg/mL phorbol 12,13-dibutyrate, and 1 µg/mL brefeldin A for 5 hours. Cells were then fixed, permeabilized, and stained with Anti-IL-2 antibodies as well as with CD154 antibodies. CD4+
cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals.