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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with PMA/ionomycin for 6 hours. After 2 hours, brefeldin A was added. Cells were then fixed, permeabilized, and intracellularly stained with Anti-GMCSF antibodies and CD4 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Gating was performed according to forward and side scatter properties of the cells.