ODN 1982

ODN 1982

Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 1982 is used as a control for CpG ODN, especially B-class CpG ODNs.

Background information

TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells
1,2
. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs
3
.
Control compounds for CpG ODNs share the sequence with their stimulatory counterparts but contain GpC dinucleotides instead of the CpG motifs.

Applications

ODN 1982 can be used as a control for CpG ODN, especially B-class CpG ODN.
  • Selected references

    1. Hemmi, H. et al. (2000) A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745
    2. Krieg, A. M. et al. (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549
    3. Bauer, S. et al. (2001) Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc. Natl. Acad. Sci. U.S.A. 98: 9237-9242
Product options: 2
130-100-104

ODN 1982

200 µg
EUR 130,00 
130-100-277

ODN 1982

1 mg
EUR 450,00