Mouse IL-4

Mouse IL-4

IL-4 stands for interleukin 4. Mouse IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-4 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH 2 cells.
  • In vitro studies of bone marrow precursor development to DCs, together with GM-CSF.
  • In vitro investigation of B cell-T cell interaction, together with CD40-Ligand.

Background information

IL-4 is produced mainly by activated T
H
2 cells and, to a lesser extent, by T
H
1 cells. It binds to the IL-4 receptor α (CD124), followed by the dimerization with other receptor chains to generate type 1 and type 2 receptors. IL-4 promotes the proliferation and differentiation of activated B cells and the expression of MHC class II antigens. Mice over expressing IL-4 have elevated levels of IgE and IgG1 and show a deficiency in T cell maturation. CD4
+
T cells from knockout mice lacking IL-4 are not able to produce T
H
2 cytokines after
in vitro
stimulation. The biological activity of IL-4 is species-specific, i.e. murine IL-4 is inactive on human cells and
vice versa.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of HT-2 cells (NIBSC 91/656)
  • premium grade: ≥ 8×
    10
    6
    U/mg
  • research grade: ≥ 5×
    10
    6
    U/mg
Product options: 6
E. coli
10 µg
EUR 135,00 
E. coli
25 µg
EUR 210,00 
E. coli
10 µg
EUR 165,00 
E. coli
25 µg
EUR 265,00 
E. coli
100 µg
EUR 525,00 
E. coli
1000 µg
EUR 2.220,00